| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR14A16 |
| Uniprot No | Q8NHC5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-309 aa |
| 活性数据 | MANLTIVTEFILMGFSTNKNMCILHSILFLLIYLCALMGNVLIIMITTLDHHLHTPVYFF LKNLSFLDLCLISVTAPKSIANSLIHNNSISFLGCVSQVFLLLSSASAELLLLTVMSFDR YTAICHPLHYDVIMDRSTCVQRATVSWLYGGLIAVMHTAGTFSLSYCGSNMVHQFFCDIP QLLAISCSENLIREIALILINVVLDFCCFIVIIITYVHVFSTVKKIPSTEGQSKAYSICL PHLLVVLFLSTGFIAYLKPASESPSILDAVISVFYTMLPPTFNPIIYSLRNKAIKVALGM LIKGKLTKK |
| 分子量 | 34.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR14A16蛋白的模拟参考文献(非真实文献，基于领域知识整合)：
---
1. **文献名称**: "Functional expression and ligand screening of human olfactory receptor OR14A16 in heterologous cell systems"
**作者**: Zhang, Y. et al.
**摘要**: 本研究通过哺乳动物细胞表达系统(如HEK293)成功实现了OR14A16的重组表达，并利用钙离子成像技术筛选其潜在气味配体，发现该受体对特定脂肪酸衍生物(如棕榈酸)表现出响应性。
---
2. **文献名称**: "Structural insights into the activation mechanism of human olfactory receptor OR14A16 via cryo-EM"
**作者**: Chéron, J.B. et al.
**摘要**: 利用冷冻电镜技术解析了OR14A16与其激动剂结合状态下的三维结构，揭示了该受体跨膜结构域的构象变化，为嗅觉受体的信号转导机制提供了分子层面的解释。
---
3. **文献名称**: "OR14A16 as a potential biomarker in chronic rhinosinusitis: Expression and functional analysis"
**作者**: Smith, T. et al.
**摘要**: 发现OR14A16在慢性鼻窦炎患者的鼻黏膜组织中表达显著上调，体外实验表明其激活可能通过MAPK通路促进炎症因子释放，提示其在嗅觉障碍相关疾病中的潜在作用。
---
4. **文献名称**: "Olfactory receptor OR14A16 modulates glucose metabolism in adipose tissue via Gαs signaling"
**作者**: Li, H. et al.
**摘要**: 首次报道OR14A16在脂肪组织中的非嗅觉功能，证明其通过G蛋白偶联信号通路调控脂联素分泌，影响葡萄糖代谢稳态，为代谢疾病治疗提供新靶点。
---
注：以上文献信息为模拟生成，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。建议结合关键词“OR14A16”、“olfactory receptor”和“recombinant protein”进一步探索。
Recombinant human OR14A16 protein is a genetically engineered form of the olfactory receptor 14A16. a class A G protein-coupled receptor (GPCR) predominantly expressed in olfactory sensory neurons. As part of the olfactory receptor (OR) family, OR14A16 is implicated in detecting odorant molecules and transducing chemical signals into cellular responses, though its specific ligands and physiological roles remain under investigation. OR14 are encoded by the largest human gene family, with ~400 functional receptors, yet most are poorly characterized due to challenges in heterologous expression and ligand identification.
The production of recombinant OR14A16 leverages molecular cloning to express the protein in vitro systems, such as HEK293 or insect cells, often requiring chaperones like RTP1/2 for proper membrane localization. This approach enables structural and functional studies, including ligand screening via calcium imaging or electrophysiology, which are critical for elucidating its activation mechanisms and odorant specificity. Recent studies suggest ORs may have extranasal roles in metabolism, cancer, and cellular signaling, spurring interest in OR14A16's potential non-olfactory functions.
Recombinant OR14A16 also serves as a tool for biomedical applications, including biosensor development or drug discovery targeting GPCR pathways. However, low natural abundance and structural complexity pose challenges, emphasizing the need for optimized expression platforms. Further research aims to decode its biological significance and therapeutic relevance, bridging gaps in sensory biology and disease mechanisms.
×
