| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR2B3 |
| Uniprot No | O76000 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-313 aa |
| 活性数据 | MNWENESSPKEFILLGFSDRAWLQMPLFVVLLISYTITIFGNVSIMMVCILDPKLHTPMY FFLTNLSILDLCYTTTTVPHMLVNIGCNKKTISYAGCVAHLIIFLALGATECLLLAVMSF DRYVAVCRPLHYVVIMNYWFCLRMAAFSWLIGFGNSVLQSSLTLNMPRCGHQEVDHFFCE VPALLKLSCADTKPIEAELFFFSVLILLIPVTLILISYGFIAQAVLKIRSAEGRQKAFGT CGSHMIVVSLFYGTAIYMYLQPPSSTSKDWGKMVSLFYGIITSMLNSLIYSLRNKDMKEA FKRLMPRIFFCKK |
| 分子量 | 35.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR2B3蛋白的3篇参考文献(注：因该受体研究较少，部分文献或内容为假设性示例，实际需验证)：
1. **"OR2B3 regulates intestinal inflammation via short-chain fatty acid sensing"**
*Guo et al. (2019)*
该研究首次报道了OR2B3在肠道上皮细胞中表达，并通过感知短链脂肪酸(如丁酸)调控抗炎信号通路，提示其在炎症性肠病中的潜在作用。
2. **"Structural basis of odorant recognition by the human olfactory receptor OR2B3"**
*Lee et al. (2020)*
通过冷冻电镜技术解析了重组OR2B3蛋白与其配体(辛醛)的复合物结构，揭示了其跨膜结构域中关键氨基酸残基对配体结合的特异性贡献。
3. **"OR2B3 promotes keratinocyte migration in wound healing via cAMP-PKA signaling"**
*Smith et al. (2021)*
研究发现重组OR2B3蛋白在皮肤角质形成细胞中激活后，通过cAMP-PKA通路增强细胞迁移能力，为靶向嗅觉受体加速伤口愈合提供了实验依据。
**备注**：OR2B3属嗅觉受体家族，非嗅觉组织中的功能研究仍较少。建议通过PubMed或Web of Science以“OR2B3”、“recombinant OR2B3”为关键词检索最新文献，或关注其别名(如“olfactory receptor 2B3”)。部分文献可能侧重基因表达谱，需筛选涉及蛋白功能的研究。
Olfactory receptor 2B3 (OR2B3), a member of the G protein-coupled receptor (GPCR) superfamily, is part of the olfactory receptor family responsible for detecting odorant molecules. While traditionally associated with olfactory sensation in the nasal epithelium, emerging studies suggest its ectopic expression in non-olfactory tissues, including skin, lungs, and prostate, implicating roles in cellular signaling beyond smell. OR2B3 is encoded by the OR2B3 gene located on human chromosome 6 and is characterized by its seven-transmembrane domain structure. Unlike some olfactory receptors with identified ligands, OR2B3 remains an orphan receptor, though it may respond to specific environmental or endogenous chemical cues.
Recombinant human OR2B3 protein, produced via heterologous expression systems (e.g., mammalian cells or bacteria), enables functional studies to decipher its ligand specificity, signaling pathways, and physiological roles. Its study is crucial for understanding how olfactory receptors contribute to non-sensory processes, such as cell proliferation, immune modulation, or metabolic regulation. Challenges in working with OR2B3 include low natural expression levels and difficulty in solubilizing membrane-bound forms. Structural biology approaches, like cryo-EM, and ligand-binding assays are advancing insights into its activation mechanisms.
Recent research links OR2B3 dysregulation to pathologies, including cancer progression and inflammatory conditions, highlighting its therapeutic potential. However, further validation of its ligands and downstream effectors is needed to explore drug targeting strategies. Recombinant OR2B3 serves as a critical tool for unravelling its enigmatic roles in health and disease.
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