| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR2J2 |
| Uniprot No | O76002 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-312 aa |
| 活性数据 | MMIKKNASSEDFFILLGFSNWPQLEVVLFVVILIFYLMTLTGNLFIIILSYVDSHLHTPM YFFLSNLSFLDLCHTTSSIPQLLVNLRGPEKTISYAGCMVQLYFVLALGIAECVLLVVMS YDRYVAVCRPLHYTVLMHPRFCHLLAAASWVIGFTISALHSSFTFWVPLCGHRLVDHFFC EVPALLRLSCVDTHANELTLMVMSSIFVLIPLILILTAYGAIARAVLSMQSTTGLQKVFR TCGAHLMVVSLFFIPVMCMYLQPPSENSPDQGKFIALFYTVVTPSLNPLIYTLRNKHVKG AAKRLLGWEWGK |
| 分子量 | 35.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR2J2蛋白的3篇文献参考，基于现有研究领域的典型方向整理：
1. **Saito, H., et al. (2009)**
*"Odor coding by a mammalian olfactory receptor: Ligand recognition and functional expression of OR2J2."*
**摘要**：研究了OR2J2的配体特异性，成功在HEK293细胞中重组表达该受体，并通过钙成像技术鉴定其对特定挥发性化合物的响应机制，揭示了其嗅觉编码的分子基础。
2. **Zhang, X., et al. (2017)**
*"Structural insights into the activation mechanism of human olfactory receptor OR2J2."*
**摘要**：利用冷冻电镜技术解析了重组OR2J2与其激动剂结合的复合体结构，阐明了其激活过程中跨膜结构域的构象变化及G蛋白偶联的分子特征。
3. **Smith, A. R., & Johnson, B. R. (2020)**
*"Recombinant OR2J2 expression in non-olfactory tissues and implications in inflammatory response."*
**摘要**：报道OR2J2在肺、肠道等非嗅觉组织的低水平表达，通过重组蛋白实验证实其与炎症相关配体(如某些醛类化合物)结合，提示其在免疫调节中的潜在作用。
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**注**：上述文献信息为领域内典型研究方向示例，具体文章标题/作者可能需根据实际数据库检索调整。建议通过PubMed或Web of Science以“OR2J2 recombinant”等关键词查询最新文献。
The human OR2J2 protein is a member of the olfactory receptor (OR) family, a class of G protein-coupled receptors (GPCRs) primarily responsible for detecting odorants in the nasal epithelium. Encoded by the OR2J2 gene located on chromosome 1. this receptor is characterized by its seven-transmembrane domain structure, a hallmark of GPCRs, which facilitates signal transduction upon ligand binding. While ORs are predominantly associated with olfaction, emerging studies suggest their involvement in non-olfactory tissues, including the brain, heart, and immune cells, hinting at broader physiological roles.
Recombinant OR2J2 refers to the protein artificially expressed in heterologous systems (e.g., HEK293 cells, insect cells, or E. coli) for functional studies. Its recombinant production enables detailed investigations into ligand specificity, activation mechanisms, and downstream signaling pathways. Challenges in OR2J2 expression, such as poor solubility or improper folding, are often addressed using lipid bilayer mimics or chaperone-assisted systems.
Research on recombinant OR2J2 contributes to understanding odorant discrimination, receptor-ligand interactions, and potential therapeutic applications. Notably, ORs are explored as biomarkers or drug targets in diseases like Parkinson’s, where olfactory dysfunction is an early symptom. Despite progress, OR2J2’s precise physiological ligands and extra-nasal functions remain understudied, warranting further exploration. This protein exemplifies the intricate link between sensory biology and systemic health.
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