| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR2F2 |
| Uniprot No | O95006 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-317 aa |
| 活性数据 | MEIDNQTWVREFILLGLSSDWCTQISLFSLFLVTYLMTVLGNCLIVLLIRLDSRLHTPMY FFLTNLSLVDVSYATSVVPQLLAHFLAEHKAIPFQSCAAQLFFSLALGGIEFVLLAVMAY DRHVAVSDRLRYSAIMHGGLCARLAITSWVSGSINSLVQTAITFQLPMCTNKFIDHISCE LLAVVRLACVDTSSNEAAIMVSSIVLLMTPFCLVLLSYIRIISTILKIQSREGRKKAFHT CASHLTVVALCYGTTIFTYIQPHSGPSVLQEKLISVFYAIVMPLLNPVIYSLRNKEVKGA WHKLLEKFSGLTSKLGT |
| 分子量 | 35.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR2F2蛋白的假设性参考文献示例(请注意，这些为模拟示例，实际文献需通过学术数据库验证)：
---
1. **文献名称**: "Functional Expression and Ligand Screening of Recombinant Human OR2F2 in a Heterologous Cell System"
**作者**: Müller A, et al.
**摘要**: 本研究在HEK293细胞中成功表达重组OR2F2蛋白，并通过钙离子荧光成像技术筛选其潜在配体，发现该受体对特定短链脂肪酸具有选择性响应，揭示了其在非嗅觉组织中的潜在生理作用。
2. **文献名称**: "Cryo-EM Structure of Recombinant OR2F2 Provides Insights into Olfactory Receptor Activation"
**作者**: Chen X, et al.
**摘要**: 通过冷冻电镜解析重组OR2F2蛋白的三维结构，首次揭示了该受体在配体结合前后的构象变化，为理解嗅觉受体信号转导机制提供结构基础。
3. **文献名称**: "OR2F2 Recombinant Protein as a Biomarker for Early-Stage Cancer Detection"
**作者**: Park H, et al.
**摘要**: 研究发现OR2F2在多种癌细胞中异常表达，利用重组OR2F2蛋白开发了抗体检测技术，初步验证其作为癌症诊断标志物的潜力。
4. **文献名称**: "Optimization of Recombinant OR2F2 Expression in Insect Cells for High-Throughput Drug Screening"
**作者**: Silva R, et al.
**摘要**: 通过杆状病毒-昆虫细胞系统优化OR2F2重组蛋白的表达与纯化流程，建立了基于该蛋白的高通量药物筛选平台，用于靶向嗅觉受体药物的开发。
---
**注意事项**：
- 以上文献为示例性内容，实际研究中可能存在差异。
- 推荐通过PubMed、Google Scholar等平台，以关键词“OR2F2 recombinant”、“OR2F2 expression”或“olfactory receptor OR2F2”检索最新文献。
- 部分研究可能侧重OR2F2的基因调控或生理功能，而非重组蛋白本身，需仔细筛选。
The recombinant human OR2F2 protein is a genetically engineered form of the olfactory receptor 2F2 (OR2F2), a member of the G protein-coupled receptor (GPCR) superfamily. Olfactory receptors (ORs) are primarily responsible for detecting odorants and mediating olfactory signaling. OR2F2. encoded by the OR2F2 gene located on chromosome 7. is a putative receptor with hypothesized roles in odorant recognition, though its specific ligands and physiological functions remain incompletely characterized. As a class A GPCR, it is predicted to have a seven-transmembrane domain structure, typical of odorant-sensing receptors.
Recombinant OR2F2 is typically produced in heterologous systems (e.g., mammalian cells, insect cells) to enable proper folding and post-translational modifications crucial for ligand-binding activity. Its production facilitates structural studies, ligand screening, and investigations into OR2F2's potential roles beyond olfaction, including possible ectopic expression in non-olfactory tissues. Research efforts focus on deciphering its activation mechanisms, downstream signaling pathways (e.g., cAMP cascade), and relevance to diseases—some ORs have been implicated in cancer progression and metabolic disorders. Challenges persist in functional characterization due to difficulties in expressing full-length functional ORs *in vitro* and identifying natural agonists. Current applications include developing OR-targeted biosensors and exploring therapeutic avenues for嗅觉 dysfunction.
×
