| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR51F2 |
| Uniprot No | Q8NH61 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-342 aa |
| 活性数据 | MTETSLSSQCFPMSVLNNTIAEPLIFLLMGIPGLKATQYWISIPFCLLYVVAVSGNSMIL FVVLCERSLHKPMYYFLSMLSATDLSLSLCTLSTTLGVFWFEAREINLNACIAQMFFLHG FTFMESGVLLAMAFDRFVAICYPLRYTTILTNARIAKIGMSMLIRNVAVMLPVMLFVKRL SFCSSMVLSHSYCYHVDLIQLSCTDNRINSILGLFALLSTTGFDCPCILLSYILIIRSVL SIASSEERRKAFNTCTSHISAVSIFYLPLISLSLVHRYGHSAPPFVHIIMANVFLLIPPV LNPIIYSVKIKQIQKAIIKVLIQKHSKSNHQLFLIRDKAIYE |
| 分子量 | 38.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR51F2蛋白的假设性参考文献，基于嗅觉受体相关研究的常见方向整理：
1. **文献名称**：*Heterologous Expression and Ligand Screening of Human Olfactory Receptor OR51F2 in HEK293 Cells*
**作者**：Smith J, et al.
**摘要**：本研究利用HEK293细胞成功表达了重组人OR51F2蛋白，并通过钙离子成像技术筛选其潜在配体，发现其对特定短链脂肪酸分子具有剂量依赖性激活作用，提示其在化学感知中的潜在作用。
2. **文献名称**：*OR51F2 Expression in Non-Olfactory Tissues and Its Role in Prostate Cancer Progression*
**作者**：Zhang Y, et al.
**摘要**：通过免疫组化与重组OR51F2蛋白体外实验，揭示OR51F2在前列腺癌细胞中异常高表达，并可能通过调控MAPK信号通路促进肿瘤侵袭，为前列腺癌治疗提供新靶点。
3. **文献名称**：*Structural Insights into OR51F2 via Cryo-EM: Implications for Odorant Recognition*
**作者**：Lee S, Park H.
**摘要**：首次解析了重组OR51F2蛋白与配体结合状态下的冷冻电镜结构，阐明了其跨膜结构域与疏水性配体的相互作用机制，为嗅觉受体激活模型提供结构依据。
4. **文献名称**：*OR51F2 as a Regulator of Adipocyte Metabolism: Recombinant Protein-Based Functional Analysis*
**作者**：Johnson R, et al.
**摘要**：使用重组OR51F2蛋白进行体外实验，发现其表达于脂肪细胞膜并通过激活AMPK通路促进脂肪酸氧化，暗示其在代谢性疾病中的潜在调控作用。
**注意**：以上为基于领域研究的模拟参考文献，实际文献需通过数据库(如PubMed、Web of Science)以“OR51F2”“recombinant OR51F2”为关键词检索获取。若研究较少，可扩展至嗅觉受体家族(如OR51家族)的重组表达及功能分析方法。
The human OR51F2 protein, a member of the olfactory receptor (OR) family, belongs to the class A G protein-coupled receptor (GPCR) superfamily. Located on chromosome 11p15.4. OR51F2 is primarily associated with olfactory perception, where it detects specific odorant molecules in the nasal epithelium. However, emerging studies suggest its extra-nasal expression in tissues like the prostate, kidneys, and testes, hinting at non-olfactory roles in cellular signaling, metabolism, or disease pathways. As a transmembrane protein, it features seven α-helical domains traversing the cell membrane, facilitating ligand binding and signal transduction via G-protein activation. Recombinant OR51F2 is typically produced using heterologous expression systems (e.g., HEK293 cells) with epitope tags for purification and detection. Its recombinant form enables structural studies (e.g., X-ray crystallography, cryo-EM), ligand screening, and functional assays to decode activation mechanisms and downstream signaling. Research on OR51F2 contributes to understanding OR evolution, tissue-specific functions, and potential therapeutic targets, particularly in cancers or metabolic disorders where ORs show aberrant expression. Challenges remain in identifying its natural ligands and physiological context, highlighting its status as an understudied receptor with untapped biomedical relevance.
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