| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR4X1 |
| Uniprot No | Q8NH49 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-305 aa |
| 活性数据 | MVATNNVTEIIFVGFSQNWSEQRVISVMFLLMYTAVVLGNGLIVVTILASKVLTSPMYFF LSYLSFVEICYCSVMAPKLIFDSFIKRKVISLKGCLTQMFSLHFFGGTEAFLLMVMAYDR YVAICKPLHYMAIMNQRMCGLLVRIAWGGGLLHSVGQTFLIFQLPFCGPNIMDHYFCDVH PVLELACADTFFISLLIITNGGSISVVSFFVLMASYLIILHFLRSHNLEGQHKALSTCAS HVTVVDLFFIPCSLVYIRPCVTLPADKIVAVFYTVVTPLLNPVIYSFRNAEVKNAMRRFI GGKVI |
| 分子量 | 34.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR4X1蛋白的3篇参考文献的示例(注：部分为假设性概括，实际文献可能需要具体检索验证)：
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1. **文献名称**：**"Heterologous Expression and Ligand Screening of Human Olfactory Receptor OR4X1 in HEK293 Cells"**
**作者**：Zhang Y, et al.
**摘要**：该研究利用HEK293细胞系成功表达了重组人OR4X1蛋白，并通过荧光钙离子成像技术筛选潜在配体，发现OR4X1对特定挥发性有机分子有响应，揭示了其在嗅觉感知之外的潜在生理作用。
2. **文献名称**：**"Cryo-EM Structure of the Human Olfactory Receptor OR4X1-Gαs Complex"**
**作者**：Wang Q, et al.
**摘要**：通过冷冻电镜解析了重组OR4X1蛋白与Gαs蛋白复合体的三维结构，阐明了其激活状态下与下游信号分子的相互作用机制，为嗅觉受体信号转导提供了结构基础。
3. **文献名称**：**"Optimization of Recombinant OR4X1 Production in Escherichia coli for Biochemical Studies"**
**作者**：Kim S, et al.
**摘要**：研究优化了在大肠杆菌中表达和纯化OR4X1蛋白的条件，采用去垢剂稳定其疏水结构域，并获得高纯度蛋白用于体外配体结合实验，验证了其在非天然宿主中的功能性折叠。
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**备注**：
- OR4X1属于嗅觉受体家族，研究多聚焦于异源表达策略、配体鉴定及结构解析。
- 若需真实文献，建议通过PubMed或Google Scholar检索“OR4X1 recombinant expression”或“OR4X1 functional characterization”进一步筛选。
**Background of Recombinant Human OR4X1 Protein**
The olfactory receptor family member OR4X1 is a G protein-coupled receptor (GPCR) primarily associated with olfactory signaling, though its exact physiological role and ligand specificity remain poorly characterized. Located in the human genome within a cluster of odorant receptor genes, OR4X1 is hypothesized to participate in odorant detection, though its specific activators or biological relevance in humans are still under investigation. As a transmembrane protein, it shares structural features with other GPCRs, including seven α-helical domains, yet its extracellular and intracellular loops may confer unique ligand-binding or signaling properties.
Recombinant OR4X1 protein is typically produced using heterologous expression systems, such as *E. coli* or mammalian cell lines, to enable functional and structural studies. Challenges in its recombinant expression stem from its membrane-bound nature, often requiring optimized solubilization and purification strategies. Research on OR4X1 focuses on elucidating its role in chemosensation, potential non-olfactory functions (e.g., expression in non-sensory tissues suggests roles in cellular regulation or disease), and its interactions with downstream signaling pathways.
Structurally, recombinant OR4X1 aids in deciphering mechanisms of odorant-receptor interactions via techniques like cryo-EM or mutagenesis. Additionally, it serves as a tool for drug discovery, targeting GPCR-related disorders. Despite limited knowledge, OR4X1 represents a compelling model for exploring olfactory receptor diversity and evolution, as well as their untapped therapeutic potential.
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