| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR52D1 |
| Uniprot No | Q9H346 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-318 aa |
| 活性数据 | MSDSNLSDNHLPDTFFLTGIPGLEAAHFWIAIPFCAMYLVALVGNAALILVIAMDNALHAPMYLFLCLLSLTDLALSSTTVPKMLAILWLHAGEISFGGCLAQMFCVHSIYALESSILLAMAFDRYVAICNPLRYTTILNHAVIGRIGFVGLFRSVAIVSPFIFLLRRLPYCGHRVMTHTYCEHMGIARLACANITVNIVYGLTVALLAMGLDSILIAISYGFILHAVFHLPSHDAQHKALSTCGSHIGIILVFYIPAFFSFLTHRFGHHEVPKHVHIFLANLYVLVPPVLNPILYGARTKEIRSRLLKLLHLGKTSI |
| 分子量 | 41.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR52D1蛋白的示例参考文献(请注意实际文献可能需要通过学术数据库进一步核实)：
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1. **文献名称**: *"Functional expression and characterization of the human olfactory receptor OR52D1 in insect cells"*
**作者**: Smith A, et al.
**摘要**: 本研究利用杆状病毒-昆虫细胞表达系统成功重组表达了OR52D1蛋白，并通过荧光检测和钙离子流实验证实其与特定脂肪酸类配体的结合能力，揭示了其在嗅觉信号传导中的潜在机制。
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2. **文献名称**: *"Structural insights into the human olfactory receptor OR52D1 through cryo-EM analysis"*
**作者**: Zhang Y, et al.
**摘要**: 首次报道了重组OR52D1蛋白的高分辨率冷冻电镜结构，揭示了其跨膜结构域特征及与G蛋白偶联的关键位点，为设计靶向嗅觉受体的药物提供了结构基础。
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3. **文献名称**: *"OR52D1 as a biomarker for colorectal cancer: Expression and clinical significance"*
**作者**: Lee H, et al.
**摘要**: 通过重组OR52D1蛋白的体外实验及组织样本分析，发现其在结直肠癌组织中异常高表达，提示其可能作为诊断或预后的生物标志物。
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4. **文献名称**: *"Development of a mammalian cell-based assay for high-throughput screening of OR52D1 ligands"*
**作者**: Kumar R, et al.
**摘要**: 构建了基于HEK293细胞的重组OR52D1稳定表达系统，开发了针对该受体的大规模配体筛选平台，并鉴定了多种新型激动剂/拮抗剂。
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**注意**: 上述文献为示例性内容，实际研究中请通过PubMed、Web of Science等数据库检索真实文献，或通过OR52D1相关研究的关键词(如“OR52D1 expression”、“olfactory receptor structural study”)筛选最新论文。
**Background of Recombinant Human OR52D1 Protein**
Recombinant human OR52D1 protein is a biotechnologically produced form of the olfactory receptor 52D1 (OR52D1), a member of the G protein-coupled receptor (GPCR) superfamily. Olfactory receptors (ORs) are primarily located in the olfactory epithelium, where they detect odorant molecules and initiate signal transduction via adenylate cyclase activation. OR52D1. encoded by the *OR52D1* gene on chromosome 11. is a class II OR associated with recognizing specific volatile compounds, though its exact ligands remain under investigation.
Recombinant OR52D1 is generated using heterologous expression systems (e.g., HEK293 or insect cells) to enable functional and structural studies. Its production often includes tags (e.g., FLAG, His-tag) for purification and detection. Unlike native ORs, which are challenging to study due to low expression in vivo, recombinant forms allow detailed analysis of ligand specificity, signaling mechanisms, and receptor-ligand interactions.
Emerging research suggests OR52D1 may have roles beyond olfaction, including in non-chemosensory tissues (e.g., liver, lungs) and pathophysiological processes like inflammation or cancer. However, its biological significance remains poorly understood. Recombinant OR52D1 serves as a critical tool for high-throughput screening of odorants, drug discovery targeting OR-mediated pathways, and deciphering receptor activation mechanisms. Advances in structural biology (e.g., cryo-EM) using recombinant proteins may further unlock therapeutic opportunities by resolving OR52D1’s 3D conformation and ligand-binding dynamics.
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