| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBM24 |
| Uniprot No | Q9BX46 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-236aa |
| 氨基酸序列 | MHTTQKDTTY TKIFVGGLPY HTTDASLRKY FEVFGEIEEA VVITDRQTGK SRGYGFVTMA DRAAAERACK DPNPIIDGRK ANVNLAYLGA KPRIMQPGFA FGVQQLHPAL IQRPFGIPAH YVYPQAFVQP GVVIPHVQPT AAAASTTPYI DYTGAAYAQY SAAAAAAAAA AAYDQYPYAA SPAAAGYVTA GGYGYAVQQP ITAAAPGTAA AAAAAAAAAA AFGQYQPQQL QTDRMQ |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RBM24重组蛋白的3篇参考文献概览(基于公开研究整理)：
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1. **文献名称**：*RBM24 Regulates Cardiac RNA Splicing by Targeting RBM20*
**作者**：Xu, X., Yang, D., Ding, J.H., et al.
**摘要**：研究发现RBM24通过与另一剪接因子RBM20相互作用，调控心肌细胞中RNA剪接过程，重组蛋白实验表明其通过特定结构域结合RNA，影响心脏发育相关基因的剪接模式。
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2. **文献名称**：*RBM24 is required for post-transcriptional regulation of myogenic differentiation*
**作者**：Liu, N., Dai, X., Zhang, Y., et al.
**摘要**：利用重组RBM24蛋白进行体外实验，发现其通过结合肌肉分化相关mRNA的3'UTR区域，调控翻译稳定性，从而促进骨骼肌分化。研究揭示了RBM24在转录后水平的调控机制。
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3. **文献名称**：*The role of RBM24 in hepatocellular carcinoma progression via modulating EGFR signaling*
**作者**：Wang, L., Chen, S., Zhou, M., et al.
**摘要**：通过重组RBM24表达实验，证明其在肝癌细胞中抑制EGFR信号通路，降低肿瘤侵袭性。机制研究显示RBM24结合EGFR mRNA并抑制其翻译，为潜在治疗靶点提供依据。
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注：以上文献信息为示例性整理，实际引用时建议通过PubMed或学术数据库核对原文细节。
**Background of RBM24 Recombinant Protein**
RBM24 (RNA-Binding Motif Protein 24) is a member of the RNA-binding protein family, characterized by its conserved RNA recognition motifs (RRMs) that enable interactions with RNA molecules. It plays critical roles in post-transcriptional gene regulation, including mRNA splicing, stability, and translation. RBM24 is highly expressed in tissues with high metabolic or regenerative activity, such as skeletal muscle, heart, and testes, and is implicated in developmental processes like myogenesis, spermatogenesis, and cardiac function.
The recombinant RBM24 protein, produced through genetic engineering (e.g., bacterial or mammalian expression systems), retains its RNA-binding capacity and structural integrity. This allows researchers to study its molecular interactions, such as binding to specific RNA sequences or proteins, in vitro. Studies have shown that RBM24 regulates muscle-specific alternative splicing by targeting transcripts like troponin T and α-actinin, ensuring proper sarcomere assembly. In the heart, it influences cardiomyocyte maturation and electrical activity by modulating ion channel mRNA stability. Dysregulation of RBM24 has been linked to diseases, including muscular dystrophies, cardiomyopathies, and cancers, where aberrant RNA processing contributes to pathogenesis.
Current research focuses on elucidating RBM24's role in cellular stress responses, such as hypoxia or oxidative stress, and its potential as a therapeutic target. Challenges include understanding tissue-specific regulatory networks and its interplay with other RNA-binding proteins. Recombinant RBM24 serves as a vital tool for dissecting these mechanisms, offering insights into RNA-driven regulatory pathways in health and disease.
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