| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | P2RY6 |
| Uniprot No | Q15077 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-328 aa |
| 活性数据 | MEWDNGTGQALGLPPTTCVYRENFKQLLLPPVYSAVLAAGLPLNICVITQICTSRRALTR TAVYTLNLALADLLYACSLPLLIYNYAQGDHWPFGDFACRLVRFLFYANLHGSILFLTCI SFQRYLGICHPLAPWHKRGGRRAAWLVCVAVWLAVTTQCLPTAIFAATGIQRNRTVCYDL SPPALATHYMPYGMALTVIGFLLPFAALLACYCLLACRLCRQDGPAEPVAQERRGKAARM AVVVAAAFAISFLPFHITKTAYLAVRSTPGVPCTVLEAFAAAYKGTRPFASANSVLDPIL FYFTQKKFRRRPHELLQKLTAKWQRQGR |
| 分子量 | 36.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟示例格式的参考文献（非真实存在，仅供格式参考）：
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1. **"Recombinant expression and functional characterization of human P2RY6 in HEK293 cells"**
*作者：Smith A, et al.*
摘要：该研究成功在HEK293细胞中重组表达人源P2RY6蛋白，验证其与Gq蛋白的偶联能力，并证实其通过UDP激活下游钙信号通路，为受体药理学研究提供工具。
2. **"P2RY6-mediated UDP signaling promotes macrophage chemotaxis in inflammatory responses"**
*作者：Zhang L, Wang H.*
摘要：通过重组P2RY6蛋白及基因敲除模型，发现P2RY6在巨噬细胞中响应UDP信号，调控趋化因子分泌和细胞迁移，揭示其在炎症性疾病中的潜在作用靶点。
3. **"Structural insights into ligand binding of recombinant human P2RY6 receptor"**
*作者：Tanaka K, et al.*
摘要：利用冷冻电镜解析重组人P2RY6蛋白与UDP结合的复合体结构，揭示关键氨基酸残基对配体识别的贡献，为选择性药物设计提供结构基础。
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**注意**：以上文献信息为模拟生成，实际研究中需通过PubMed、Web of Science等数据库查询真实文献。如需具体论文推荐，建议结合近期研究领域（如神经炎症、代谢调控等）筛选关键词。
P2RY6, a member of the G protein-coupled receptor (GPCR) family, is encoded by the P2RY6 gene in humans. It selectively binds to uridine diphosphate (UDP), a nucleotide acting as an extracellular signaling molecule. As a purinergic receptor, P2RY6 is primarily coupled to Gq proteins, activating phospholipase C (PLC)-mediated pathways to mobilize intracellular calcium, though it may also engage Gi/o signaling under specific conditions. This receptor is broadly expressed in tissues, including immune cells, the nervous system, and epithelial tissues, playing roles in inflammation, neurotransmission, and secretory processes.
Research highlights P2RY6's involvement in pathological conditions. It modulates immune responses by regulating cytokine release in macrophages and microglia, contributing to neuroinflammation and chronic inflammatory diseases. In oncology, P2RY6 overexpression has been linked to tumor progression in certain cancers through pathways promoting cell proliferation and survival. Additionally, it influences metabolic disorders by affecting insulin secretion and adipocyte function.
Recombinant human P2RY6 protein, typically produced in mammalian expression systems like HEK293 cells, retains native receptor conformation and functionality. This engineered protein serves as a critical tool for drug discovery, enabling high-throughput screening of agonists/antagonists, structural studies of UDP-binding mechanisms, and investigation of receptor dimerization or desensitization processes. Current research focuses on developing P2RY6-targeted therapies for conditions ranging from inflammatory bowel disease to glioblastoma, while also exploring its crosstalk with other purinergic receptors in complex disease pathways.
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