| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | P2RX2 |
| Uniprot No | Q9UBL9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-404 aa |
| 活性数据 | MAAAQPKYPAGATARRLARGCWSALWDYETPKVIVVRNRRLGVLYRAVQLLILLYFVWYVFIVQKSYQESETGPESSIITKVKGITTSEHKVWDVEEYVKPPEGGSVFSIITRVEATHSQTQGTCPESIRVHNATCLSDADCVAGELDMLGNGLRTGRCVPYYQGPSKTCEVFGWCPVEDGASVSQFLGTMAPNFTILIKNSIHYPKFHFSKGNIADRTDGYLKRCTFHEASDLYCPIFKLGFIVEKAGESFTELAHKGGVIGVIINWDCDLDLPASECNPKYSFRRLDPKHVPASSGYNFRFAKYYKINGTTTRTLIKAYGIRIDVIVHGQAGKFSLIPTIINLATALTSVGVGSFLCDWILLTFMNKNKVYSHKKFDKMVDTPASEPAQASTPTDPKGLAQL |
| 分子量 | 71.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人P2RX2（P2X2受体）蛋白的典型文献摘要概括：
1. **文献名称**: *Purification and functional reconstitution of the recombinant P2X2 receptor*
**作者**: Torres GE, Egan TM, Voigt MM
**摘要**: 该研究利用昆虫细胞表达系统成功纯化了重组人P2X2受体蛋白，并通过脂质体重组证明了其对ATP的配体门控离子通道活性，揭示了该受体的基础电生理特性。
2. **文献名称**: *Structural insights into the human P2X2 receptor by cryo-EM*
**作者**: Mansoor SE, et al.
**摘要**: 通过冷冻电镜技术解析了重组人P2X2受体在开放和关闭状态下的高分辨率结构，阐明了ATP结合后诱导的构象变化机制，为开发靶向P2X受体的药物提供依据。
3. **文献名称**: *Disease-associated mutations in the human P2X2 receptor alter ATP sensitivity*
**作者**: Castro A, et al.
**摘要**: 本研究利用HEK293细胞表达重组P2X2受体，发现耳聋相关突变体（如p.Leu12Pro）会显著改变其对ATP的敏感性，揭示了P2X2功能异常与听觉障碍的关联。
*注：以上文献为虚构示例，实际研究中可检索PubMed或Web of Science获取真实文献（如检索词：recombinant human P2X2 receptor）。*
P2RX2 (P2X purinoceptor 2) is a member of the P2X receptor family, a group of ligand-gated ion channels activated by extracellular ATP. It plays a critical role in cellular signaling, particularly in sensory and neuronal systems. Structurally, P2RX2 forms a trimeric channel with two transmembrane domains per subunit, facilitating ion permeation (primarily Na⁺, K⁺, and Ca²⁺) upon ATP binding to its extracellular domain. This receptor is implicated in diverse physiological processes, including auditory transduction, neuropathic pain modulation, and inflammatory responses. Dysregulation of P2RX2 has been linked to hearing loss, neurodegenerative disorders, and chronic pain conditions.
Recombinant human P2RX2 protein is engineered for experimental studies to elucidate its structural and functional mechanisms. Produced using heterologous expression systems (e.g., HEK293 or CHO cells), the recombinant protein retains native conformational and electrophysiological properties, enabling in vitro analyses like patch-clamp recordings or calcium flux assays. Its purification often involves affinity tags (e.g., His-tag) followed by chromatography. Research applications include drug screening for ATP antagonists, investigating ATP-mediated signaling pathways, and developing therapies targeting P2X receptor-related pathologies. Studies using recombinant P2RX2 have advanced understanding of ATP-gated ion channel dynamics and their therapeutic potential.
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