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Recombinant Human P2RY8 Protein

  • 中文名: 重组人(P2RY8)蛋白
  • 别    名: P2RY8
货号: PAX2000-10083
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点P2RY8
Uniprot NoQ86VZ1
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-359 aa
活性数据MQVPNSTGPDNATLQMLRNPAIAVALPVVYSLVAAVSIPGNLFSLWVLCRRMGPRSPSVIFMINLSVTDLMLASVLPFQIYYHCNRHHWVFGVLLCNVVTVAFYANMYSSILTMTCISVERFLGVLYPLSSKRWRRRRYAVAACAGTWLLLLTALSPLARTDLTYPVHALGIITCFDVLKWTMLPSVAMWAVFLFTIFILLFLIPFVITVACYTATILKLLRTEEAHGREQRRRAVGLAAVVLLAFVTCFAPNNFVLLAHIVSRLFYGKSYYHVYKLTLCLSCLNNCLDPFVYYFASREFQLRLREYLGCRRVPRDTLDTRRESLFSARTTSVRSEAGAHPEGMEGATRPGLQRQESVF
分子量40.6 kDa
蛋白标签His tag N-Terminus
缓冲液0
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.


参考文献



以下是3篇虚拟的关于重组人P2RY8蛋白的研究文献示例(非真实发表):  


1. **"Structural Insights into Human P2RY8 G Protein-Coupled Receptor Activation"**  

   *作者:Zhang, L., et al. (2020)*  

   摘要:通过冷冻电镜解析了重组人P2RY8蛋白与其天然配体结合的活性构象,揭示了该受体激活下游G蛋白信号的结构基础,为靶向P2RY8的药物设计提供了理论依据。  


2. **"P2RY8 Regulates Immune Cell Migration in Lymphoma Microenvironments"**  

   *作者:Müller, C., et al. (2018)*  

   摘要:研究证明重组人P2RY8蛋白在B细胞淋巴瘤中异常高表达,其信号通路通过调控趋化因子释放影响肿瘤相关巨噬细胞的迁移,可能成为免疫治疗的潜在靶点。  


3. **"Recombinant P2RY8 Expression and Functional Characterization in Airway Epithelial Cells"**  

   *作者:Harrison, R., et al. (2021)*  

   摘要:构建了重组人P2RY8蛋白的稳定表达系统,发现其在气道上皮细胞中参与调控炎症因子的分泌,提示P2RY8在慢性呼吸道疾病(如哮喘)中的病理作用。  


4. **"Ligand Screening of P2RY8 Using a Novel Bioluminescence Resonance Energy Transfer Assay"**  

   *作者:Tanaka, K., et al. (2019)*  

   摘要:开发基于重组人P2RY8蛋白的BRET高通量筛选平台,鉴定出多个新型小分子激动剂/拮抗剂,为探索该受体在代谢疾病中的功能提供工具化合物。  


注:以上文献为示例,实际研究请参考PubMed、Web of Science等数据库的权威发表。


背景信息



Recombinant human P2RY8 protein is a purified, biologically active form of the P2Y receptor family member 8, a G-protein-coupled receptor (GPCR) encoded by the *P2RY8* gene. This receptor binds extracellular nucleotides such as adenosine triphosphate (ATP) and adenosine diphosphate (ADP), acting as a key mediator in purinergic signaling pathways. P2RY8 is prominently expressed in immune cells, including lymphocytes and macrophages, and plays roles in immune regulation, cell proliferation, and inflammatory responses. Its activation triggers intracellular signaling cascades via Gαi/o proteins, modulating processes like chemotaxis, cytokine release, and apoptosis. Notably, P2RY8 has been implicated in cancer biology, particularly in lymphoid malignancies, where chromosomal translocations involving *P2RY8* are linked to aberrant activation of oncogenic pathways. Recombinant P2RY8 is typically produced in mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications and ligand-binding functionality. It serves as a critical tool for studying receptor-ligand interactions, drug discovery, and signaling mechanisms in immunological and oncological contexts. Current research focuses on its therapeutic potential as a target for immune disorders and cancers, leveraging its role in modulating tumor microenvironments and immune cell behavior. The recombinant protein’s stability and activity are validated through assays like cAMP inhibition, calcium flux, and cell migration studies.


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