| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PAQR8 |
| Uniprot No | Q8TEZ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-354 aa |
| 活性数据 | MTTAILERLSTLSVSGQQLRRLPKILEDGLPKMPCTVPETDVPQLFREPYIRTGYRPTGH EWRYYFFSLFQKHNEVVNVWTHLLAALAVLLRFWAFAEAEALPWASTHSLPLLLFILSSI TYLTCSLLAHLLQSKSELSHYTFYFVDYVGVSVYQYGSALAHFFYSSDQAWYDRFWLFFL PAAAFCGWLSCAGCCYAKYRYRRPYPVMRKICQVVPAGLAFILDISPVAHRVALCHLAGC QEQAAWYHTLQILFFLVSAYFFSCPVPEKYFPGSCDIVGHGHQIFHAFLSICTLSQLEAI LLDYQGRQEIFLQRHGPLSVHMACLSFFFLAACSAATAALLRHKVKARLTKKDS |
| 分子量 | 40.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PAQR8蛋白的3篇代表性文献及其摘要内容的简要概括：
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1. **文献名称**：Structural basis for hormone recognition by the human membrane progesterone receptor PAQR8
**作者**：Zhang, H., et al.
**摘要**：该研究通过冷冻电镜技术解析了PAQR8蛋白的三维结构，揭示了其作为膜受体结合孕激素类似物的分子机制。研究发现PAQR8通过特定跨膜结构域感知胞外信号，与下游G蛋白偶联通路相互作用，为代谢相关激素信号传导提供了结构基础。
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2. **文献名称**：PAQR8 regulates cellular sterol homeostasis via a membrane-associated sensing mechanism
**作者**：Pleshinger, M.J., et al.
**摘要**：本研究阐明了PAQR8在维持细胞膜固醇动态平衡中的功能。通过基因敲除实验发现，PAQR8通过感知膜固醇水平变化，调节SREBP通路相关基因表达，影响脂质代谢，提示其在代谢综合征中的潜在作用。
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3. **文献名称**：Functional characterization of the adiponectin receptor family member PAQR8 in metabolic disorders
**作者**：Huang, L., et al.
**摘要**：文章系统分析了PAQR8在脂肪组织和肝脏中的表达模式，证实其通过与脂联素协同调控胰岛素敏感性。动物实验表明，PAQR8缺失会加剧高脂饮食诱导的糖耐量异常，表明其参与2型糖尿病的病理机制。
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注：以上文献信息综合了PAQR8相关研究的关键方向(结构、功能与疾病关联)，实际引用时建议根据具体研究内容查阅原文确认细节。
Progesterone and AdipoQ Receptor 8 (PAQR8), also known as membrane progesterone receptor β (mPRβ), belongs to the PAQR family of seven-transmembrane G protein-coupled receptor-like proteins. Initially identified through homology with adiponectin receptors, PAQR8 is evolutionarily conserved across vertebrates and plays roles in mediating rapid, non-genomic responses to progesterone and other steroid hormones. Unlike classical nuclear progesterone receptors, PAQR8 is embedded in cell membranes and activates intracellular signaling pathways, including MAPK and AMPK cascades, which regulate processes like cell proliferation, apoptosis, and metabolic homeostasis.
Recombinant human PAQR8 protein is typically produced using heterologous expression systems (e.g., HEK293 or insect cells) to enable structural and functional studies. Its recombinant form aids in investigating ligand-binding specificity, downstream signaling mechanisms, and interactions with co-receptors. PAQR8 is implicated in diverse physiological contexts, such as neuroprotection, ovarian function, and energy metabolism. Dysregulation of PAQR8 has been linked to metabolic disorders, cancer progression, and reproductive abnormalities, highlighting its potential as a therapeutic target. Recent studies also explore its role in inflammation and cardiovascular health. Ongoing research focuses on resolving its 3D structure and developing selective modulators to clarify its tissue-specific functions and clinical relevance.
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