| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHA5 |
| Uniprot No | Q9Y5H7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 183-289 aa |
| 活性数据 | TNEEETNFLELVLRKSLDREETQEHRLLVIATDGGKPELTGTVQLLINVLDANDNAPEFDKSIYNVRLLENAPSGTLVIKLNASDADEGINKEIVYFFSNLVLDDVK |
| 分子量 | 37.51 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PCDHA5蛋白的参考文献示例(注：以下内容为基于领域知识的模拟文献，实际文献可能需要通过数据库进一步检索确认)：
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1. **标题**: *"Recombinant expression and functional characterization of human PCDHA5 in neuronal adhesion"*
**作者**: Smith J, et al.
**摘要**: 本研究在大肠杆菌系统中成功表达并纯化了重组人PCDHA5胞外结构域，证实其通过同源结合介导细胞间黏附，并调控神经元突触的可塑性。
2. **标题**: *"Structural insights into the PCDHA5 ectodomain and its role in synaptic connectivity"*
**作者**: Lee S, et al.
**摘要**: 通过X射线晶体学解析了重组人PCDHA蛋白的胞外区结构，揭示了其钙离子依赖性同源二聚化机制，并发现其突变体与自闭症谱系障碍的潜在关联。
3. **标题**: *"PCDHA5 regulates axon guidance through recombinant protein-mediated signaling pathways"*
**作者**: Chen R, et al.
**摘要**: 在HEK293细胞中表达重组人PCDHA5.发现其与神经导向分子Netrin-1相互作用，激活下游信号通路，影响轴突导向和神经元迁移。
4. **标题**: *"Proteolytic processing and oligomerization of recombinant PCDHA5 in vitro"*
**作者**: Tanaka K, et al.
**摘要**: 研究重组人PCDHA5在体外的蛋白酶切割位点及其多聚体形成特性，表明其可能通过剪切后修饰参与细胞表面识别。
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**备注**：若需具体文献，建议通过PubMed或Google Scholar以“recombinant PCDHA5”、“PCDHA5 protein function”等关键词检索。部分研究可能集中在原钙黏蛋白家族(如PCDHA簇)整体分析，PCDHA5可能作为其中之一被提及。
Protocadherin alpha-5 (PCDHA5), a member of the clustered protocadherin-alpha (PCDHA) family, is a transmembrane protein involved in cell-cell adhesion and neural circuit formation. It plays a crucial role in establishing neuronal diversity and connectivity during brain development. The PCDHA cluster contains 15 variable exons (PCDHA1-PCDHA14 and a constant exon), with PCDHA5 encoding a cadherin-like extracellular domain, a transmembrane region, and a conserved cytoplasmic tail. Its extracellular domain mediates homophilic interactions, promoting selective recognition and adhesion between neurons, which is essential for axonal guidance, synaptic specificity, and neuronal survival.
As a neural-specific protein, PCDHA5 is predominantly expressed in the central nervous system. Dysregulation of PCDHA5 has been linked to neurodevelopmental disorders, including autism spectrum disorders and schizophrenia, as well as some cancers. Recombinant human PCDHA5 protein, produced via engineered cell lines, is widely used to study its structural properties, binding partners, and signaling pathways in vitro. Researchers employ it to investigate mechanisms underlying neural wiring, synaptic plasticity, and disease-related mutations. Its potential therapeutic applications include targeting neurodegenerative conditions or malignancies associated with protocadherin dysfunction, though further functional studies are needed to elucidate its precise roles.
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