| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PGBD4 |
| Uniprot No | Q96DM1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-585 aa |
| 活性数据 | MSNPRKRSIP MRDSNTGLEQ LLAEDSFDES DFSEIDDSDN FSDSALEADK IRPLSHLESD GKSSTSSDSG RSMKWSARAM IPRQRYDFTG TPGRKVDVSD ITDPLQYFEL FFTEELVSKI TRETNAQAAL LASKPPGPKG FSRMDKWKDT DNDELKVFFA VMLLQGIVQK PELEMFWSTR PLLDTPYLRQ IMTGERFLLL FRCLHFVNNS SISAGQSKAQ ISLQKIKPVF DFLVNKFSTV YTPNRNIAVD ESLMLFKGPL AMKQYLPTKR VRFGLKLYVL CESQSGYVWN ALVHTGPGMN LKDSADGLKS SRIVLTLVND LLGQGYCVFL DNFNISPMLF RELHQNRTDA VGTARLNRKQ IPNDLKKRIA KGTTVARFCG ELMALKWCDG KEVTMLSTFH NDTVIEVNNR NGKKTKRPRV IVDYNENMGA VDSADQMLTS YPSERKRHKV WYKKFFHHLL HITVLNSYIL FKKDNPEHTM SHINFRLALI ERMLEKHHKP GQQHLRGRPC SDDVTPLRLS GRHFPKSIPA TSGKQNPTGR CKICCSQYDK DGKKIRKETR YFCAECDVPL CVVPCFEIYH TKKNY |
| 分子量 | 67.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人PGBD4蛋白的参考文献示例(注：内容为示例性虚构，实际文献请通过学术数据库核实):
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1. **Structural Insights into Human PGBD4 Transposase-like Protein**
*Authors: Matheson, T.J., et al. (2020)*
摘要：通过X射线晶体学解析了PGBD4蛋白的催化核心结构，揭示其转座酶活性所需的保守结构域，并证实其体外切割特定DNA序列的能力。
2. **PGBD4-Mediated Genomic Rearrangements in Hematopoietic Cells**
*Authors: Reyes, L.M., et al. (2021)*
摘要：研究发现PGBD4在急性淋巴细胞白血病中异常高表达，通过诱导基因组DNA双链断裂，促进致癌性染色体重排，CRISPR敲除可抑制癌细胞增殖。
3. **Biochemical Characterization of PGBD4 DNA-Binding Specificity**
*Authors: Zhang, Y., et al. (2019)*
摘要：体外实验表明PGBD4优先识别富含CTTA基序的DNA区域，结合时诱导DNA弯曲，提示其通过构象变化调控染色质可及性，影响基因表达。
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建议通过 **PubMed/Google Scholar** 检索真实文献时使用关键词：
`PGBD4 OR "piggyBac transposase derivative 4"` + `structure/mechanism/cancer`
PGBD4 (piggyBac transposable element-derived protein 4) is a human protein encoded by the *PGBD4* gene, belonging to the piggyBac transposase-derived protein family. These proteins are evolutionarily related to DNA transposases but have lost their ancestral transposition activity in vertebrates. PGBD4 contains conserved domains resembling the piggyBac transposase, including a predicted catalytic domain with a DDE integrase motif and zinc-binding regions, suggesting potential DNA-binding or nuclease functions. However, its precise biochemical activity remains unresolved.
Recent studies propose roles in genome stability and somatic genome rearrangements. PGBD4 exhibits nuclear localization and interacts with chromatin-associated proteins. Intriguingly, it shares structural homology with RAG1. a lymphoid-specific recombinase critical for V(D)J recombination, though functional overlap remains unconfirmed. The protein shows tissue-specific expression patterns, with elevated levels observed in lymphoid tissues and certain cancers. Evidence suggests potential involvement in cancer-related genomic instability, with *PGBD4* overexpression correlating with chromosomal abnormalities in human tumors. Current research focuses on elucidating its molecular mechanisms, particularly its capacity to recognize or process DNA substrates, and its possible contribution to oncogenesis through genome rearrangement pathways. Despite progress, PGBD4's physiological functions and regulation in normal and disease contexts require further characterization.
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