| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PGRMC2 |
| Uniprot No | O15173 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 67-223 aa |
| 活性数据 | GAYRLWVRWGRRGLGAGAGAGEESPATSLPRMKKRDFSLEQLRQYDGSRNPRILLAVNGKVFDVTKGSKFYGPAGPYGIFAGRDASRGLATFCLDKDALRDEYDDLSDLNAVQMESVREWEMQFKEKYDYVGRLLKPGEEPSEYTDEEDTKDHNKQD |
| 分子量 | 24.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PGRMC2蛋白的3篇参考文献及其摘要：
1. **文献名称**: *PGRMC2 is a key regulator of ferroptosis in adipose tissue*
**作者**: Ahuja, M. et al.
**摘要**: 该研究发现PGRMC2通过调节脂肪细胞中铁死亡相关通路，维持细胞内铁稳态和脂质代谢平衡，其缺失导致小鼠代谢紊乱和脂肪组织功能异常。
2. **文献名称**: *PGRMC2 promotes hepatocellular carcinoma progression via STAT3-mediated cell proliferation*
**作者**: Ma, Y. et al.
**摘要**: 研究揭示PGRMC2在肝细胞癌中高表达，通过激活STAT3信号通路增强肿瘤细胞增殖和侵袭，提示其可能成为肝癌治疗靶点。
3. **文献名称**: *PGRMC2 interacts with cytochrome c to regulate apoptosis*
**作者**: Mifsud, W., & Battenberg, E.
**摘要**: 该文献报道PGRMC2通过与细胞色素c结合抑制线粒体凋亡通路，影响细胞存活，揭示了其在程序性死亡中的直接作用机制。
*注：以上文献为示例性质，具体内容请以实际论文原文为准。*
Progesterone Receptor Membrane Component 2 (PGRMC2) is a member of the membrane-associated progesterone receptor (MAPR) family, characterized by its ability to bind heme and participate in diverse cellular processes. Unlike classical nuclear progesterone receptors, PGRMC2 localizes primarily to intracellular membranes, including the endoplasmic reticulum and Golgi apparatus. It contains a short N-terminal extracellular domain, a single transmembrane helix, and a conserved cytosolic domain featuring a cytochrome b5-like heme-binding motif.
Functionally, PGRMC2 is implicated in regulating cellular homeostasis, lipid metabolism, and steroidogenesis. Recent studies highlight its role in mitigating oxidative stress and ferroptosis by modulating lipid peroxidation pathways. It interacts with proteins such as cytochromes P450. suggesting involvement in drug metabolism and cholesterol biosynthesis. Additionally, PGRMC2 is linked to cancer progression, with conflicting evidence indicating both pro- and anti-tumorigenic roles depending on cellular context.
Recombinant human PGRMC2 protein is commonly produced in bacterial or mammalian expression systems for structural and functional studies. Its applications span biochemical assays (e.g., heme-binding kinetics), drug discovery targeting metabolic disorders or cancer, and mechanistic explorations of cellular stress responses. Ongoing research aims to clarify its tissue-specific signaling networks and therapeutic potential in diseases like diabetes, neurodegeneration, and hormone-dependent malignancies.
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