| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PHKG2 |
| Uniprot No | P15735 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-406 aa |
| 活性数据 | MTLDVGPEDE LPDWAAAKEF YQKYDPKDVI GRGVSSVVRR CVHRATGHEF AVKIMEVTAE RLSPEQLEEV REATRRETHI LRQVAGHPHI ITLIDSYESS SFMFLVFDLM RKGELFDYLT EKVALSEKET RSIMRSLLEA VSFLHANNIV HRDLKPENIL LDDNMQIRLS DFGFSCHLEP GEKLRELCGT PGYLAPEILK CSMDETHPGY GKEVDLWACG VILFTLLAGS PPFWHRRQIL MLRMIMEGQY QFSSPEWDDR SSTVKDLISR LLQVDPEARL TAEQALQHPF FERCEGSQPW NLTPRQRFRV AVWTVLAAGR VALSTHRVRP LTKNALLRDP YALRSVRHLI DNCAFRLYGH WVKKGEQQNR AALFQHRPPG PFPIMGPEEE GDSAAITEDE AVLVLG |
| 分子量 | 46.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PHKG2蛋白的推测文献示例(注：文献信息为模型虚拟生成，仅供参考，实际文献请通过学术数据库查询)：
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1. **文献名称**：*Functional Characterization of Recombinant Human PHKG2 in Glycogen Phosphorylase Regulation*
**作者**：Gentry, M.S., et al.
**摘要**：该研究成功在大肠杆菌中表达了重组人PHKG2蛋白，并证明其在磷酸化酶激酶复合物中的催化作用，揭示了PHKG2通过钙调蛋白依赖性机制激活糖原磷酸化酶的分子机制。
2. **文献名称**：*Structural Insights into PHKG2 Mutants Associated with Liver Glycogen Storage Disease*
**作者**：Hudson, J.W., et al.
**摘要**：通过重组PHKG2蛋白的晶体结构解析，研究发现了与肝糖贮积症相关的PHKG2突变体(如p.R225Q)对蛋白构象的影响，并验证了突变导致酶活性丧失的分子基础。
3. **文献名称**：*Expression and Purification of Active Human PHKG2 in a Baculovirus System*
**作者**：DePaoli-Roach, A.A., et al.
**摘要**：利用杆状病毒-昆虫细胞系统高效表达重组PHKG2.结合质谱分析验证了其翻译后修饰(如磷酸化)对磷酸化酶激酶全酶活性的调控作用。
4. **文献名称**：*PHKG2-Deficient Mice and Rescue Strategies Using Recombinant Protein Therapy*
**作者**：Feng, Y., & Irimia, J.M.
**摘要**：构建了PHKG2敲除小鼠模型，通过重组PHKG2蛋白的体外递送实验，证明其对糖原代谢异常的治疗潜力，为基因治疗提供理论基础。
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**提示**：实际文献建议通过**PubMed**或**Google Scholar**搜索关键词：*"PHKG2 recombinant protein"*、*"PHKG2 structure/function"*，或关联疾病如*"glycogen storage disease type 9"*。
**Background of Recombinant Human PHKG2 Protein**
Phosphorylase Kinase Gamma 2 (PHKG2) is a catalytic subunit of the phosphorylase kinase (PHK) complex, which plays a critical role in glycogen metabolism. As a member of the calcium/calmodulin-dependent protein kinase (CAMK) family, PHKG2 regulates the activation of glycogen phosphorylase, an enzyme essential for glycogen breakdown into glucose-1-phosphate to meet cellular energy demands. The PHK holoenzyme consists of four subunits (α, β, γ, δ), with PHKG2 representing the gamma-2 isoform, predominantly expressed in the liver and skeletal muscle.
PHKG2 activity is tightly controlled by calcium signaling and phosphorylation events, linking glycogenolysis to hormonal and neural stimuli. Mutations in the *PHKG2* gene are associated with glycogen storage disease type IX (GSD IX), characterized by hepatomegaly, growth retardation, and intermittent hypoglycemia due to impaired glycogen breakdown. This underscores its physiological importance in energy homeostasis.
Recombinant human PHKG2 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*) to study its structure, enzymatic mechanisms, and interactions. It serves as a vital tool for investigating metabolic disorders, drug discovery targeting glycogen-related diseases, and elucidating calcium-dependent signaling pathways. Additionally, recombinant PHKG2 aids in functional studies to validate pathogenic mutations and develop therapeutic strategies for GSD IX and related conditions. Its application extends to in vitro assays for kinase activity screening and biomarker research, highlighting its multifaceted role in biomedical research.
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