| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PHF21B |
| Uniprot No | Q96EK2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-531 aa |
| 活性数据 | MELQSRPEAL AVELARHQNG DLKKQLHERQ PRIAALSDKQ ALGTITAVPV TGPQVSSLQR LAGQGAAVLP QVRPKTLIPD SLPVAPGRDR PPKQPPTFQK ATVVSVKNPS PALPTANNTV SHVPAPGSQP QALAEPAALA SPLSSAGVAY AIISTSPSNA AAMAPSTAVS VVSDSIKVQP LLISADNKPP PRLLSSPHPA THHCPLHPSS LPLTPPSPSL SPSPLHGIFQ VIIIQPQVQT QPESTAESRP PTEEPSQGAQ ATKKKKEDRP PTQENPEKIA FMVALGLVTT EHLEEIQSKR QERKRRSTAN PAYSGLLETE RKRLASNYLN NPLFLTARAN EDPCWKNEIT HDEHCAACKR GANLQPCGTC PGAYHLSCLE PPLKTAPKGV WVCPRCQQKA LKKDEGVPWT GMLAIVHSYV THKTVKEEEK QKLLQRGSEL QNEHQQLEER DRRLASAVQK CLELKTSLLA RQRGTQSSLD RLRALLRLIQ GEQLLQVTMT TTSPAPLLAG PWTKPSVAAT HPTVQHPQGH N |
| 分子量 | 57.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人PHF21B蛋白相关的参考文献及其摘要概述：
1. **文献名称**: *PHF21B is a subunit of the LSD1-CoREST-HDAC complex and targets neurodevelopmental genes*
**作者**: Yang, M. et al.
**摘要**: 本研究揭示PHF21B作为LSD1-CoREST-HDAC表观遗传调控复合物的组成蛋白，参与通过组蛋白去甲基化抑制神经元分化相关基因的表达。实验使用重组PHF21B蛋白验证了其与LSD1的相互作用及其在染色质重塑中的功能。
2. **文献名称**: *Structural insights into PHF21B recognition by the BHC complex*
**作者**: Gupta, R. et al.
**摘要**: 通过X射线晶体学分析重组人PHF21B蛋白结构，揭示了其N端PHD结构域特异性识别组蛋白H3K4me0修饰，并阐明了PHF21B在BHC复合物中调控基因沉默的结构基础。
3. **文献名称**: *PHF21B mutations link chromatin dysregulation to autism spectrum disorders*
**作者**: Lee, J. et al.
**摘要**: 报道PHF21B基因突变与自闭症相关，体外实验发现重组突变型PHF21B蛋白破坏与CoREST的相互作用，导致下游靶基因(如BDNF)异常表达，提示其在神经发育中的关键作用。
4. **文献名称**: *Recombinant PHF21B purification and functional analysis in stem cell pluripotency*
**作者**: Chen, X. et al.
**摘要**: 描述大肠杆菌表达系统高效纯化重组PHF21B蛋白的方法，并证明其通过维持多能性基因(如OCT4)的低甲基化状态，促进胚胎干细胞自我更新。
注：以上文献信息为基于领域知识的示例，实际文献需通过PubMed或Web of Science检索确认，部分内容可能结合了PHF21B的已知功能(如与BHC复合物关联)。
**Background of Recombinant Human PHF21B Protein**
PHF21B (PHD Finger Protein 21B) is a chromatin-associated protein belonging to the PHD zinc finger protein family, characterized by the presence of a plant homeodomain (PHD) that recognizes histone modifications. It plays roles in epigenetic regulation, transcriptional control, and chromatin remodeling. PHF21B interacts with histone-modifying enzymes and transcriptional complexes, influencing gene expression programs critical for neurodevelopment, cell differentiation, and synaptic plasticity.
Studies link PHF21B to neurological disorders, including intellectual disability, autism spectrum disorders, and schizophrenia, often via dysregulation of neurodevelopmental pathways. It is implicated in modulating histone H3 lysine 4 methylation (H3K4me2/3), a mark associated with active transcription, suggesting its role in maintaining neuronal gene networks.
Recombinant human PHF21B protein, produced using bacterial or mammalian expression systems, retains functional domains for studying molecular interactions. It is utilized to investigate its binding partners, enzymatic activity, and role in chromatin dynamics. Research applications include elucidating PHF21B's contribution to disease mechanisms, screening therapeutic compounds, and exploring its potential as a biomarker. Structural analyses (e.g., crystallography) further reveal how its PHD domain engages with histone tails or other epigenetic regulators.
Overall, PHF21B represents a key molecular bridge between chromatin states and neural function, with broad implications for understanding neuropathologies and developing targeted therapies.
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