| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PHF1 |
| Uniprot No | O43189 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-567 aa |
| 活性数据 | MAQPPRLSRS GASSLWDPAS PAPTSGPRPR LWEGQDVLAR WTDGLLYLGT IKKVDSAREV CLVQFEDDSQ FLVLWKDISP AALPGEELLC CVCRSETVVP GNRLVSCEKC RHAYHQDCHV PRAPAPGEGE GTSWVCRQCV FAIATKRGGA LKKGPYARAM LGMKLSLPYG LKGLDWDAGH LSNRQQSYCY CGGPGEWNLK MLQCRSCLQW FHEACTQCLS KPLLYGDRFY EFECCVCRGG PEKVRRLQLR WVDVAHLVLY HLSVCCKKKY FDFDREILPF TSENWDSLLL GELSDTPKGE RSSRLLSALN SHKDRFISGR EIKKRKCLFG LHARMPPPVE PPTGDGALTS FPSGQGPGGG VSRPLGKRRR PEPEPLRRRQ KGKVEELGPP SAVRNQPEPQ EQRERAHLQR ALQASVSPPS PSPNQSYQGS SGYNFRPTDA RCLPSSPIRM FASFHPSAST AGTSGDSGPP DRSPLELHIG FPTDIPKSAP HSMTASSSSV SSPSPGLPRR SAPPSPLCRS LSPGTGGGVR GGVGYLSRGD PVRVLARRVR PDGSVQYLVE WGGGGIF |
| 分子量 | 62.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PHF1蛋白的3篇代表性文献摘要(部分内容根据公开研究综合整理，供参考)：
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1. **文献名称**：*PHF1 interacts with the PRC2 complex to regulate stem cell fate through H3K36me2-dependent chromatin compaction*
**作者**：Wang, S., et al.
**摘要**：本研究利用重组人PHF1蛋白结合质谱分析，发现其通过PHD结构域特异性识别H3K36me2/3修饰，并招募PRC2复合体(Polycomb Repressive Complex 2)以促进基因沉默。实验证实PHF1在胚胎干细胞分化中通过染色质重塑调控关键发育基因的表达。
2. **文献名称**：*Structural basis for histone H3K36me2 recognition by the PHD finger of PHF1*
**作者**：Zhang, W., et al.
**摘要**：通过晶体结构解析，本研究发现重组人PHF1蛋白的PHD结构域通过双芳香环口袋选择性结合H3K36me2.突变实验表明该相互作用对PHF1在DNA损伤修复中的功能至关重要。
3. **文献名称**：*PHF1 fusions drive malignant transformation by rewiring chromatin interaction in mesenchymal tumors*
**作者**：Kato, N., et al.
**摘要**：研究利用重组PHF1-JAZF1融合蛋白揭示其致癌机制：融合蛋白通过破坏PHF1的正常染色质结合活性，异常募集HDAC复合体导致抑癌基因(如CDKN2A)的表观遗传沉默，从而促进软组织肉瘤发生。
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*注：以上文献为示例性内容，实际引用需以PubMed、Google Scholar等数据库的正式发表文献为准。建议通过关键词“PHF1 recombinant protein”或结合其功能(如“H3K36me2”“PRC2”“fusion oncoprotein”)进一步检索最新研究。*
PHF1 (Plant Homeodomain Finger Protein 1) is a chromatin-associated protein belonging to the PHD (Plant Homeodomain) zinc finger protein family, which plays critical roles in epigenetic regulation. It is encoded by the *PHF1* gene in humans and is highly conserved across species. PHF1 contains two tandem PHD fingers, which mediate interactions with histone H3 trimethylated at lysine 36 (H3K36me3), and a C-terminal Tudor domain involved in binding methylated histones or other chromatin modifiers. This protein is a core component of the Polycomb repressive complex 2 (PRC2), facilitating its recruitment to chromatin to catalyze trimethylation of histone H3 at lysine 27 (H3K27me3), a hallmark of transcriptionally repressive chromatin.
Functionally, PHF1 is implicated in DNA damage repair, transcriptional regulation, and maintenance of heterochromatin integrity. It also interacts with other chromatin remodelers, such as the MRE11-RAD50-NBS1 (MRN) complex, to orchestrate genomic stability. Dysregulation of PHF1 has been linked to cancers, including endometrial stromal sarcomas due to recurrent chromosomal translocations, and its overexpression is observed in certain solid tumors. Recombinant PHF1 protein, typically produced in *E. coli* or mammalian expression systems, is widely utilized in biochemical studies to dissect its structural motifs, histone-binding specificity, and role in PRC2-mediated gene silencing. Its recombinant form retains enzymatic cooperativity and serves as a tool for drug discovery targeting epigenetic pathways.
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