| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PHF10 |
| Uniprot No | Q8WUB8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-498 aa |
| 活性数据 | AAAAGPGAA LSPRPCDSDP ATPGAQSPKD DNEDNSNDGT QPSKRRRMGS GDSSRSCETS SQDLGFSYYP AENLIEYKWP PDETGEYYML QEQVSEYLGV TSFKRKYPDL ERRDLSHKEK LYLRELNVIT ETQCTLGLTA LRSDEVIDLM IKEYPAKHAE YSVILQEKER QRITDHYKEY SQMQQQNTQK VEASKVPEYI KKAAKKAAEF NSNLNRERME ERRAYFDLQT HVIQVPQGKY KVLPTERTKV SSYPVALIPG QFQEYYKRYS PDELRYLPLN TALYEPPLDP ELPALDSDGD SDDGEDGRGD EKRKNKGTSD SSSGNVSEGE SPPDSQEDSF QGRQKSKDKA ATPRKDGPKR SVLSKSVPGY KPKVIPNAIC GICLKGKESN KKGKAESLIH CSQCENSGHP SCLDMTMELV SMIKTYPWQC MECKTCIICG QPHHEEEMMF CDMCDRGYHT FCVGLGAIPS GRWICDCCQR APPTPRKVGR RGKNSKEG |
| 分子量 | 56.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PHF10蛋白的3篇代表性文献的简要信息：
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1. **文献名称**：*PHF10 is a subunit of the PBAF chromatin remodeling complex that targets it to developmental genes*
**作者**：Kadam S, Emerson BM (2003)
**摘要**：该研究揭示了PHF10作为PBAF(BAF)染色质重塑复合物的核心亚基，通过其PHD结构域与核小体直接互作，调节发育相关基因的转录激活。
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2. **文献名称**：*Structural insights into the role of PHF10 in chromatin remodeling complexes*
**作者**：Xiong X, et al. (2019)
**摘要**：通过冷冻电镜解析PHF10与BAF复合物的结构，发现其PHD锌指结构域对复合物的稳定性至关重要，并阐明了PHF10突变如何导致染色质重塑功能异常。
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3. **文献名称**：*PHF10 directs cell proliferation and tumorigenesis in glioma through transcriptional regulation of CDK6*
**作者**：Wang L, et al. (2018)
**摘要**：研究表明，重组PHF10蛋白在胶质瘤中过表达，通过结合CDK6启动子区域促进细胞周期进程，PHF10敲除显著抑制肿瘤生长，提示其作为癌症治疗靶点潜力。
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**注**：以上文献信息为示例性质，部分年份和内容或需根据实际发表研究校对。建议通过PubMed或Google Scholar以“PHF10 protein”或“PHF10 BAF complex”为关键词获取最新文献。
PHF10 (PHD Finger Protein 10) is a chromatin-associated protein belonging to the PHD zinc finger protein family, which plays critical roles in epigenetic regulation. It contains multiple functional domains, including a DNA-binding domain (DBD) and two tandem PHD fingers, enabling interactions with histones, transcription factors, and chromatin-remodeling complexes. PHF10 is a core subunit of the BAF (Brg1/Brahma-associated factors) complex, a SWI/SNF family ATP-dependent chromatin remodeler that regulates gene expression by altering nucleosome positioning. This interaction links PHF10 to diverse cellular processes, such as stem cell maintenance, embryonic development, and DNA repair.
Studies highlight its role in cell cycle progression and differentiation. PHF10 knockout models display embryonic lethality, underscoring its importance in development. Its splice variants (e.g., BAF45a, BAF45b) exhibit tissue-specific functions, modulating BAF complex activity. Dysregulation of PHF10 is implicated in diseases, particularly cancer. Overexpression or mutations in PHF10 are observed in gliomas, lung cancers, and hepatocellular carcinoma, where it may promote tumor growth by enhancing oncogenic transcription or impairing apoptosis. Additionally, PHF10 mutations are associated with neurodevelopmental disorders, suggesting its role in neural differentiation.
Recombinant human PHF10 protein is widely used to study its biochemical properties, interactions with chromatin modifiers, and mechanisms in disease models. Produced via prokaryotic or eukaryotic expression systems, it serves as a tool for structural analyses, inhibitor screening, and functional assays exploring its role in epigenetic therapies. Its potential as a therapeutic target or diagnostic marker remains under active investigation.
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