| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PHACTR1 |
| Uniprot No | Q9C0D0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-580 aa |
| 活性数据 | MDYPKMDYFL DVESAHRLLD VESAQRFFYS QGAQARRATL LLPPTLMAAS SEDDIDRRPI RRVRSKSDTP YLAEARISFN LGAAEEVERL AAMRSDSLVP GTHTPPIRRR SKFANLGRIF KPWKWRKKKS EKFKHTSAAL ERKISMRQSR EELIKRGVLK EIYDKDGELS ISNEEDSLEN GQSLSSSQLS LPALSEMEPV PMPRDPCSYE VLQPSDIMDG PDPGAPVKLP CLPVKLSPPL PPKKVMICMP VGGPDLSLVS YTAQKSGQQG VAQHHHTVLP SQIQHQLQYG SHGQHLPSTT GSLPMHPSGC RMIDELNKTL AMTMQRLESS EQRVPCSTSY HSSGLHSGDG VTKAGPMGLP EIRQVPTVVI ECDDNKENVP HESDYEDSSC LYTREEEEEE EDEDDDSSLY TSSLAMKVCR KDSLAIKLSN RPSKRELEEK NILPRQTDEE RLELRQQIGT KLTRRLSQRP TAEELEQRNI LKPRNEQEEQ EEKREIKRRL TRKLSQRPTV EELRERKILI RFSDYVEVAD AQDYDRRADK PWTRLTAADK AAIRKELNEF KSTEMEVHEL SRHLTRFHRP |
| 分子量 | 66.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **文献名称**：*PHACTR1 regulates vascular pathology via actin polymerization*
**作者**：Smith A, et al.
**摘要**：研究发现PHACTR1通过与肌动蛋白结合调控内皮细胞骨架重组，影响动脉粥样硬化斑块形成，敲除后显著减少小鼠血管炎症及斑块面积。
2. **文献名称**：*Role of PHACTR1 in cerebral cavernous malformations*
**作者**：Zhang Y, et al.
**摘要**：PHACTR1与CCM复合物(KRIT1/CCM2)相互作用，调控脑血管内皮细胞迁移及血管发育缺陷，突变导致斑马鱼模型血管畸形。
3. **文献名称**：*PHACTR1 inhibits cancer metastasis through actin dynamics modulation*
**作者**：Lee H, et al.
**摘要**：PHACTR1在乳腺癌中作为肿瘤抑制因子，通过抑制RhoA/ROCK通路减少细胞侵袭和转移，低表达与患者预后不良相关。
4. **文献名称**：*PHACTR1 modulates nuclear transport and gene transcription*
**作者**：Garcia J, et al.
**摘要**：PHACTR1通过核质穿梭调控HIF-1α等转录因子活性，影响缺血缺氧条件下的细胞存活与血管生成信号通路。
提示：上述文献为示例，实际文献需通过PubMed/Google Scholar以关键词“PHACTR1 recombinant protein”或“PHACTR1 function”检索获取。
PHACTR1 (Phosphatase and Actin Regulator 1) is a multifunctional protein encoded by the *PHACTR1* gene in humans, belonging to the phosphatase and actin regulator family. It plays a critical role in modulating cytoskeletal dynamics, cell motility, and intracellular signaling pathways by interacting with actin and protein phosphatase 1 (PP1). Structurally, PHACTR1 contains conserved RPEL domains that facilitate actin binding and nuclear localization signals, enabling its shuttling between the cytoplasm and nucleus. It regulates actin polymerization and stress fiber formation, influencing endothelial cell function, vascular development, and neuronal morphology.
PHACTR1 is highly expressed in vascular endothelial cells, smooth muscle cells, and neurons. Genome-wide association studies link *PHACTR1* genetic variants to cardiovascular diseases (e.g., coronary artery disease, hypertension), fibromuscular dysplasia, and migraine. Dysregulation of PHACTR1 impacts vascular tone, inflammation, and endothelial barrier integrity, contributing to pathological conditions. Recombinant human PHACTR1 protein, produced via bacterial or mammalian expression systems, is essential for studying its biochemical interactions, structural properties, and role in disease mechanisms. It serves as a tool for exploring therapeutic strategies targeting cytoskeletal disorders or vascular pathologies. Current research focuses on elucidating its dual regulatory functions in phosphatase activity and actin remodeling, as well as its potential as a diagnostic biomarker or drug target.
×
