| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PHACTR3 |
| Uniprot No | Q96KR7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-559 aa |
| 活性数据 | MAASEDGSGC LVSRGRSQSD PSVLTDSSAT SSADAGENPD EMDQTPPARP EYLVSGIRTP PVRRNSKLAT LGRIFKPWKW RKKKNEKLKQ TTSALEKKMA GRQGREELIK KGLLEMMEQD AESKTCNPDG GPRSVQSEPP TPKSETLTSE DAQPGSPLAT GTDQVSLDKP LSSAAHLDDA AKMPSASSGE EADAGSLLPT TNELSQALAG ADSLDSPPRP LERSVGQLPS PPLLPTPPPK ASSKTTKNVT GQATLFQASS MKSADPSLRG QLSTPTGSPH LTTVHRPLPP SRVIEELHRA LATKHRQDSF QGRESKGSPK KRLDVRLSRT SSVERGKERE EAWSFDGALE NKRTAAKESE ENKENLIINS ELKDDLLLYQ DEEALNDSII SGTLPRKCKK ELLAVKLRNR PSKQELEDRN IFPRRTDEER QEIRQQIEMK LSKRLSQRPA VEELERRNIL KQRNDQTEQE ERREIKQRLT RKLNQRPTVD ELRDRKILIR FSDYVEVAKA QDYDRRADKP WTRLSAADKA AIRKELNEYK SNEMEVHASS KHLTRFHRP |
| 分子量 | 62.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PHACTR3蛋白的3篇代表性参考文献示例(文献为虚拟概括，需进一步验证)：
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1. **文献名称**：*"PHACTR3 regulates endothelial cell migration and angiogenesis through interaction with actin cytoskeleton"*
**作者**：Zhang Y, et al.
**摘要**：研究揭示了PHACTR3通过与细胞骨架蛋白(如α-actinin)相互作用，调控内皮细胞迁移和血管生成的过程。重组PHACTR3蛋白的功能缺失实验显示其参与VEGF信号通路，提示其在血管疾病中的潜在作用。
2. **文献名称**：*"Structural and functional analysis of the PHACTR3 protein in cancer cell proliferation"*
**作者**：Smith J, et al.
**摘要**：通过重组PHACTR3蛋白的体外表达和结构解析，发现其C端结构域具有磷酸酶调控活性。研究证实PHACTR3在乳腺癌细胞中的过表达与细胞周期异常和增殖增强相关。
3. **文献名称**：*"PHACTR3 modulates synaptic plasticity via actin dynamics in neuronal development"*
**作者**：Gupta S, et al.
**摘要**：该研究表明，重组PHACTR3蛋白在小鼠海马神经元中调控突触可塑性和树突形态发生，其功能依赖于对肌动蛋白动力学的调节，为神经发育障碍提供了分子机制线索。
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**注意事项**：
1. 以上文献为示例，实际研究中请通过**PubMed**或**Google Scholar**以关键词"PHACTR3 recombinant protein"或"PHACTR3 function"检索最新文献。
2. 建议结合具体研究方向筛选，如细胞骨架、疾病模型或信号通路相关论文。
**Background of Recombinant Human PHACTR3 Protein**
Phosphatase and Actin Regulator 3 (PHACTR3), also known as scapinin, is a member of the PHACTR family that modulates cytoskeletal dynamics and cellular signaling. It contains multiple RPEL motifs, enabling interactions with actin and other regulatory proteins. PHACTR3 binds to protein phosphatase 1 (PP1), influencing dephosphorylation processes and actin filament organization. This interaction links PHACTR3 to critical cellular functions, including cell migration, proliferation, and apoptosis.
Studies suggest PHACTR3 is ubiquitously expressed, with roles in vascular development, neuronal differentiation, and stress response. Dysregulation of PHACTR3 is implicated in cancers, cardiovascular diseases, and neurodegenerative disorders, highlighting its therapeutic potential.
Recombinant human PHACTR3 (rPHACTR3) is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties and mechanisms. Purified rPHACTR3 serves as a tool for investigating PP1-mediated pathways, actin remodeling, and disease-related molecular networks. Its recombinant form enables high-throughput studies, structural analysis, and drug screening, advancing our understanding of PHACTR3’s role in health and disease.
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