| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PHC2 |
| Uniprot No | Q8IXK0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-323 aa |
| 活性数据 | MTSGNGNSASSIAGTAPQNGENKPPQAIVKPQILTHVIEGFVIQEGAEPFPVGRSSLLVGNLKKKYAQGFLPEKLPQQDHTTTTDSEMEEPYLQESKEEGAPLKLKCELCGRVDFAYKFKRSKRFCSMACAKRYNVGCTKRVGLFHSDRSKLQKAGAATHNRRRASKASLPPLTKDTKKQPTGTVPLSVTAALQLTHSQEDSSRCSDNSSYEEPLSPISASSSTSRRRQGQRDLELPDMHMRDLVGMGHHFLPSEPTKWNVEDVYEFIRSLPGCQEIAEEFRAQEIDGQALLLLKEDHLMSAMNIKLGPALKIYARISMLKDS |
| 分子量 | 62.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PHC2蛋白的3条参考文献示例(注：由于实际文献可能有限，以下内容基于相关领域研究综合虚构，仅供参考)：
1. **文献名称**：*PHC2 Mediates Polycomb Repressive Complex 1 (PRC1) Assembly via its RING Domain*
**作者**：Smith A, et al.
**摘要**：本文解析了重组表达的人PHC2蛋白在PRC1复合体组装中的作用，证实其通过RING结构域与其他Polycomb蛋白互作，参与表观遗传调控和染色质重塑。
2. **文献名称**：*Recombinant Human PHC2 Facilitates DNA Damage Response through Ubiquitination Signaling*
**作者**：Chen L, et al.
**摘要**：研究利用重组PHC2蛋白揭示了其在DNA损伤修复中的作用机制，表明PHC2通过调控泛素化修饰促进E3连接酶复合物的功能，影响细胞周期检查点激活。
3. **文献名称**：*Structural Insights into PHC2’s Role in Chromatin Compaction*
**作者**：Wang Y, et al.
**摘要**：通过重组PHC2蛋白的体外实验及冷冻电镜技术，阐明了PHC2蛋白在染色质压缩中的构象变化，揭示其与组蛋白H2A的相互作用对基因沉默的关键影响。
如需具体文献，建议通过PubMed或Web of Science以“PHC2”、“recombinant protein”等关键词检索真实研究。
**Background of Recombinant Human PHC2 Protein**
The Polyhomeotic Homolog 2 (PHC2) protein is a crucial component of the Polycomb Repressive Complex 1 (PRC1), which plays a pivotal role in epigenetic regulation of gene expression, particularly in gene silencing. PHC2 contributes to maintaining transcriptional repression by facilitating chromatin compaction and modifying histones, such as through the monoubiquitination of histone H2A at lysine 119 (H2AK119ub). This activity is essential for stable gene silencing during development, stem cell maintenance, and cellular differentiation.
Structurally, PHC2 contains a sterile alpha motif (SAM) domain, enabling self-polymerization and interaction with other PRC1 subunits, thereby stabilizing chromatin architecture. Dysregulation of PHC2 has been implicated in various cancers, where abnormal expression correlates with tumor progression, metastasis, or therapy resistance, particularly in certain solid tumors and hematological malignancies.
Recombinant human PHC2 protein is produced using expression systems (e.g., bacterial, insect, or mammalian cells) to enable functional studies. Its purified form allows researchers to investigate PRC1 assembly, chromatin interactions, and downstream regulatory mechanisms. Additionally, it serves as a tool for screening therapeutic agents targeting epigenetic dysregulation. However, challenges remain in fully recapitulating its native post-translational modifications in vitro. Continued research on PHC2 aims to clarify its context-dependent roles in health and disease, highlighting its potential as a biomarker or therapeutic target.
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