| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PHC3 |
| Uniprot No | Q8NDX5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-151 aa |
| 活性数据 | MAEAEFKDHSTAMDTEPNPGTSSVSTTTSSTTTTTITTSSSRMQQPQISVYSGSDRHAVQVIQQALHRPPSSAAQYLQQMYAAQQQHLMLHTAALQQQHLSSSQLQSLAAVQASLSSGRPSTSPTGSVTQQSSMSQTSVSSLNFFFLDLKF |
| 分子量 | 42.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PHC3蛋白的假设性参考文献示例(仅供示例参考，非真实文献)：
1. **文献名称**: "Structural and functional analysis of recombinant human PHC3 in polycomb repressive complex 1"
**作者**: Smith A, et al.
**摘要**: 研究利用重组表达技术获得了高纯度人PHC3蛋白，通过X射线晶体学解析其结构，并证实其通过N端α-螺旋参与多梳抑制复合体(PRC1)的组装，调控染色质凝缩过程。
2. **文献名称**: "Recombinant PHC3 expression in HEK293 cells: implications for epigenetic regulation"
**作者**: Zhang L, et al.
**摘要**: 开发了基于HEK293细胞的重组PHC3蛋白表达系统，分析其与Ring1B的相互作用，发现PHC3通过结合H2A泛素化标记增强基因沉默功能，提示其在癌症表观遗传失调中的作用。
3. **文献名称**: "PHC3 knockdown and recombinant rescue assay reveals its role in neural stem cell differentiation"
**作者**: Tanaka K, et al.
**摘要**: 通过重组PHC3蛋白回补实验，验证了PHC3通过调控Sox2基因的染色质状态维持神经干细胞的自我更新能力，为神经发育疾病提供了分子机制依据。
4. **文献名称**: "A scalable purification method for recombinant human PHC3 and its biochemical characterization"
**作者**: Müller R, et al.
**摘要**: 提出了一种基于大肠杆菌的重组PHC3蛋白高效纯化方案，优化了可溶性表达条件，并通过体外染色质结合实验证实PHC3的C端无序区域对DNA结合活性至关重要。
注：以上文献为模拟示例，实际需通过数据库(如PubMed、Web of Science)检索真实文献。
Recombinant human PHC3 (Polycomb homolog 3) protein is a key component of the Polycomb Repressive Complex 1 (PRC1), which plays a crucial role in epigenetic regulation and maintenance of transcriptional silencing. PHC3 belongs to the Polycomb group (PcG) family of proteins, conserved evolutionarily for chromatin modification and cell fate determination. Structurally, it contains a sterile alpha motif (SAM) domain that facilitates protein oligomerization and promotes chromatin compaction, as well as a conserved PHD domain involved in histone binding.
PHC3 contributes to PRC1-mediated gene silencing by promoting mono-ubiquitination of histone H2A (H2AK119ub), a hallmark of transcriptionally repressive chromatin. This activity influences processes like stem cell differentiation, genomic imprinting, and X-chromosome inactivation. Dysregulation of PHC3 has been associated with developmental disorders and cancers, as aberrant PRC1 function can disrupt cellular identity and proliferation.
Recombinant PHC3 is typically produced in Escherichia coli or mammalian expression systems, enabling studies on its biochemical properties, interactions, and role in chromatin remodeling. Its availability supports research into Polycomb-related disease mechanisms and potential therapeutic strategies targeting epigenetic pathways. Advances in structural and functional analyses of PHC3 continue to elucidate its contributions to epigenetic memory and cellular plasticity.
×
