| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PHF15 |
| Uniprot No | Q9NQC1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-790 aa |
| 活性数据 | MEEKRRKYSI SSDNSDTTDS HATSTSASRC SKLPSSTKSG WPRQNEKKPS EVFRTDLITA MKIPDSYQLS PDDYYILADP WRQEWEKGVQ VPAGAEAIPE PVVRILPPLE GPPAQASPSS TMLGEGSQPD WPGGSRYDLD EIDAYWLELI NSELKEMERP ELDELTLERV LEELETLCHQ NMARAIETQE GLGIEYDEDV VCDVCRSPEG EDGNEMVFCD KCNVCVHQAC YGILKVPTGS WLCRTCALGV QPKCLLCPKR GGALKPTRSG TKWVHVSCAL WIPEVSIGCP EKMEPITKIS HIPASRWALS CSLCKECTGT CIQCSMPSCV TAFHVTCAFD HGLEMRTILA DNDEVKFKSF CQEHSDGGPR NEPTSEPTEP SQAGEDLEKV TLRKQRLQQL EEDFYELVEP AEVAERLDLA EALVDFIYQY WKLKRKANAN QPLLTPKTDE VDNLAQQEQD VLYRRLKLFT HLRQDLERVR NLCYMVTRRE RTKHAICKLQ EQIFHLQMKL IEQDLCRGLS TSFPIDGTFF NSWLAQSVQI TAENMAMSEW PLNNGHREDP APGLLSEELL QDEETLLSFM RDPSLRPGDP ARKARGRTRL PAKKKPPPPP PQDGPGSRTT PDKAPKKTWG QDAGSGKGGQ GPPTRKPPRR TSSHLPSSPA AGDCPILATP ESPPPLAPET PDEAASVAAD SDVQVPGPAA SPKPLGRLRP PRESKVTRRL PGARPDAGMG PPSAVAERPK VSLHFDTETD GYFSDGEMSD SDVEAEDGGV QRGPREAGAE EVVRMGVLAS |
| 分子量 | 87.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PHF15蛋白的3篇参考文献示例(文献为虚拟示例，实际引用需核实)：
1. **文献名称**：*PHF15 regulates chromatin remodeling through interaction with the NuRD complex*
**作者**：Zhang Y, et al.
**摘要**：本研究利用重组人PHF15蛋白，发现其通过与NuRD复合体相互作用参与染色质重构，调控基因转录，揭示了其在表观遗传调控中的关键作用。
2. **文献名称**：*Recombinant human PHF15 expression and structural characterization of its PHD domain*
**作者**：Lee S, et al.
**摘要**：报道了在大肠杆菌中高效表达重组人PHF15蛋白的方法，并利用X射线晶体学解析了其PHD结构域的立体结构，为功能研究奠定基础。
3. **文献名称**：*PHF15 modulates oncogenic signaling in glioblastoma via histone methylation*
**作者**：Wang X, et al.
**摘要**：通过体外实验表明，重组PHF15蛋白可结合组蛋白H3K4me2修饰，影响胶质母细胞瘤中促癌基因的激活，提示其作为潜在治疗靶点。
注：以上文献信息为模拟生成，实际研究需通过数据库(如PubMed、Web of Science)以“PHF15”或“JADE2”为关键词检索最新文献。
PHF15 (PHD Finger Protein 15), also known as JADE3. is a member of the Polycomb-like protein family involved in epigenetic regulation. It contains a conserved plant homeodomain (PHD) zinc finger motif, which typically mediates interactions with post-translationally modified histones. PHF15 is recognized as a component of chromatin-modifying complexes, particularly the PRC2 (Polycomb Repressive Complex 2), where it contributes to histone H3K27 methylation—a key epigenetic mark associated with transcriptional repression. Studies suggest its role in recruiting PRC2 to specific genomic loci, thereby regulating gene silencing during cellular differentiation and development. Additionally, PHF15 interacts with other chromatin remodelers, linking it to DNA repair and chromatin stability. Recombinant human PHF15 protein is commonly expressed in systems like *E. coli* or mammalian cells, often fused with tags (e.g., His, GST) for purification. This protein serves as a critical tool for investigating chromatin dynamics, enzymatic assays, and probing PHF15’s interaction networks. Dysregulation of PHF15 has been implicated in cancers, including neuroblastoma and glioblastoma, where its overexpression correlates with tumor progression and poor prognosis. Ongoing research explores its potential as a therapeutic target in epigenetic diseases and malignancies.
×
