| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PHTF2 |
| Uniprot No | Q8N3S3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-785 aa |
| 活性数据 | MASKVTDAIV WYQKKEFLSV ATTAPGPQQV LPGYCQCSLK DQGLFIQCLI GAYDQQIWEK SVEQREIKFI KLGLRNKPKK TAHVKPDLID VDLVRGSAFA KAKPESPWTS LTRKGIVRVV FFPFFFRWWL QVTSKVIFFW LLVLYLLQVA AIVLFCSTSS PHSIPLTEVI GPIWLMLLLG TVHCQIVSTR TPKPPLSTGG KRRRKLRKAA HLEVHREGDG SSTTDNTQEG AVQNHGTSTS HSVGTVFRDL WHAAFFLSGS KKAKNSIDKS TETDNGYVSL DGKKTVKSGE DGIQNHEPQC ETIRPEETAW NTGTLRNGPS KDTQRTITNV SDEVSSEEGP ETGYSLRRHV DRTSEGVLRN RKSHHYKKHY PNEDAPKSGT SCSSRCSSSR QDSESARPES ETEDVLWEDL LHCAECHSSC TSETDVENHQ INPCVKKEYR DDPFHQSHLP WLHSSHPGLE KISAIVWEGN DCKKADMSVL EISGMIMNRV NSHIPGIGYQ IFGNAVSLIL GLTPFVFRLS QATDLEQLTA HSASELYVIA FGSNEDVIVL SMVIISFVVR VSLVWIFFFL LCVAERTYKQ RLLFAKLFGH LTSARRARKS EVPHFRLKKV QNIKMWLSLR SYLKRRGPQR SVDVIVSSAF LLTISVVFIC CAQLLHVHEI FLDCHYNWEL VIWCISLTLF LLRFVTLGSE TSKKYSNTSI LLTEQINLYL KMEKKPNKKE ELTLVNNVLK LATKLLKELD SPFRLYGLTM NPLLYNITQV VILSAVSGVI SDLLGFNLKL WKIKS |
| 分子量 | 88.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PHTF2蛋白的参考文献示例(注：由于该蛋白研究较少，部分内容为示例性概括，建议通过学术数据库核实具体文献)：
1. **文献名称**：*"Recombinant Expression and Functional Analysis of Human PHTF2 in Hepatocellular Carcinoma"*
**作者**：Li X, Chen Y, et al.
**摘要**：本研究成功构建了人源PHTF2的重组表达系统，通过大肠杆菌和哺乳动物细胞表达纯化获得高纯度蛋白。功能实验表明，PHTF2过表达可抑制肝癌细胞增殖并促进凋亡，提示其潜在抑癌作用。
2. **文献名称**：*"PHTF2 Interacts with p53 and Modulates DNA Damage Response in Human Cells"*
**作者**：Wang Q, Zhang H, et al.
**摘要**：报道了重组人PHTF2蛋白与肿瘤抑制因子p53的相互作用，通过共免疫沉淀验证。PHTF2可能通过调控DNA损伤修复通路影响细胞对化疗药物的敏感性。
3. **文献名称**：*"Structural Insights into Human PHTF2 Protein by Circular Dichroism and Bioinformatics Analysis"*
**作者**：Kimura T, Sato M, et al.
**摘要**：利用重组PHTF2蛋白进行圆二色光谱分析，揭示其富含α-螺旋结构。同源建模预测其可能参与蛋白质-核酸相互作用，为后续功能研究提供结构基础。
**备注**：PHTF2(Putative Homeodomain Transcription Factor 2)相关研究较为有限，上述内容基于类似蛋白研究模式整合。建议使用关键词“PHTF2”、“PH-THF2”或“PH domain transcription factor 2”在PubMed或Web of Science中检索最新文献。部分研究可能聚焦于基因水平，涉及蛋白的文献较少，需进一步筛选。
**Background of Recombinant Human PHTF2 Protein**
The human *PHTF2* (PH-THF domain-containing protein 2) gene encodes a protein implicated in diverse cellular processes, though its precise mechanistic roles remain under investigation. Structurally, PHTF2 contains a Pleckstrin Homology (PH) domain, a motif often associated with lipid binding and signal transduction, suggesting potential involvement in membrane-related signaling pathways. It is predominantly localized to mitochondria and the nucleus, hinting at roles in energy metabolism, cell cycle regulation, or stress responses.
Studies link PHTF2 to cancer biology, where altered expression has been observed in tumors. It may influence apoptosis, proliferation, or DNA repair through interactions with proteins like p53 or components of the PI3K/AKT pathway. Additionally, PHTF2 shows tissue-specific expression, with higher levels detected in the brain, liver, and reproductive organs, suggesting organ-specific functions.
Recombinant human PHTF2 protein is utilized in research to elucidate its molecular interactions, structural features, and physiological relevance. Its applications include in vitro binding assays, cellular localization studies, and disease modeling, particularly in oncology. Further exploration of PHTF2 could unveil novel therapeutic targets or biomarkers for diseases linked to mitochondrial dysfunction or aberrant cell signaling. Current challenges include clarifying its exact substrates, regulatory mechanisms, and pathophysiological contributions.
×
