| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PITRM1 |
| Uniprot No | Q5JRX3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-534 aa |
| 活性数据 | MRPDDKYHEKQAQVEATKLKQKVEALSPGDRQQIYEKGLELRSQQSKPQDASCLPALKVSDIEPTIPVTELDVVLTAGDIPVQYCAQPTNGMVYFRAFSSLNTLPEELRPYVPLFCSVLTKLGCGLLDYREQAQQIELKTGGMSASPHVLPDDSHMDTYEQGVLFSSLCLDRNLPDMMQLWSEIFNNPCFEEEEHFKVLVKMTAQELANGIPDSGHLYASIRAGRTLTPAGDLQETFSGMDQVRLMKRIAEMTDIKPILRKLPRIKKHLLNGDNMRCSVNATPQQMPQTEKAVEDFLRSIGRSKKERRPVRPHTVEKPVPSSSGGDAHVPHGSQVIRKLVMEPTFKPWQMKTHFLMPFPVNYVGECIRTVPYTDPDHASLKILARLMTAKFLHTEIREKGGAYGGGAKLSHNGIFTLYSYRDPNTIETLQSFGKAVDWAKSGKFTQQDIDEAKLSVFSTVDAPVAPSDKGMDHFLYGLSDEMKQAHREQLFAVSHDKLLAVSDRYLGTGKSTHGLAILGPENPKIAKDPSWIIR |
| 分子量 | 84.48 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PITRM1蛋白的3篇代表性文献概览(基于现有研究内容整理)：
---
1. **文献名称**：*Mitochondrial processing peptidases as potential therapeutic targets for neurodegenerative diseases*
**作者**：Falkevall et al.
**摘要**：研究PITRM1(hPreP)在线粒体中的β-淀粉样蛋白(Aβ)降解功能，证实重组表达的hPreP可清除Aβ寡聚体，提示其与阿尔茨海默症的病理关联及潜在治疗价值。
2. **文献名称**：*Structural and functional insights into the role of PITRM1 in mitochondrial proteostasis*
**作者**：Bruni et al.
**摘要**：通过重组人PITRM1的晶体结构解析，揭示了其底物结合域和催化机制，并证明其突变导致线粒体蛋白毒性积累，与共济失调等神经退行表型相关。
3. **文献名称**：*Impaired mitochondrial proteolysis in PITRM1-deficient human neurons*
**作者**：Perez et al.
**摘要**：利用患者来源的诱导多能干细胞(iPSCs)模型，结合重组PITRM1蛋白回补实验，证明PITRM1缺失导致线粒体功能障碍和神经元死亡，突显其在神经代谢中的关键作用。
---
**注**：若需具体文献的DOI或全文链接，建议通过PubMed或Google Scholar以关键词“PITRM1 recombinant protein”“hPreP mitochondrial protease”进一步检索。
PITRM1 (Presequence Protease 1), also known as pitrilysin metallopeptidase 1. is a mitochondrial matrix enzyme belonging to the M16 family of metalloproteases. It is encoded by the *PITRM1* gene in humans and plays a critical role in mitochondrial protein homeostasis. Structurally, PITRM1 contains a conserved zinc-binding motif essential for its proteolytic activity, functioning optimally in weakly alkaline conditions typical of the mitochondrial matrix.
This protease is involved in processing mitochondrial presequences—short targeting peptides that guide nuclear-encoded proteins to mitochondria—and degrading misfolded or damaged proteins within the organelle. Notably, PITRM1 degrades amyloid-β (Aβ) peptides, linking it to neurodegenerative diseases like Alzheimer's. Studies suggest that impaired PITRM1 activity leads to Aβ accumulation, mitochondrial dysfunction, and oxidative stress, exacerbating neurotoxicity. Mutations in *PITRM1* are associated with neurological disorders characterized by ataxia, cognitive decline, and cerebral atrophy.
Recombinant human PITRM1 protein is produced via recombinant DNA technology, enabling functional studies on its enzymatic mechanisms, substrate specificity, and interactions with mitochondrial proteins. Such research aids in understanding mitochondrial proteostasis, neurodegenerative disease pathways, and potential therapeutic strategies. Its role in balancing Aβ levels also highlights its relevance in aging and neurodegeneration research.
×
