| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PITPNC1 |
| Uniprot No | Q9UKF7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-332 aa |
| 活性数据 | MLLKEYRICM PLTVDEYKIG QLYMISKHSH EQSDRGEGVE VVQNEPFEDP HHGNGQFTEK RVYLNSKLPS WARAVVPKIF YVTEKAWNYY PYTITEYTCS FLPKFSIHIE TKYEDNKGSN DTIFDNEAKD VEREVCFIDI ACDEIPERYY KESEDPKHFK SEKTGRGQLR EGWRDSHQPI MCSYKLVTVK FEVWGLQTRV EQFVHKVVRD ILLIGHRQAF AWVDEWYDMT MDDVREFERA TQEATNKKIG IFPPAISISS IPLLPSSVRS APSSAPSTPL STDAPEFLSV PKDRPRKKSA PETLTLPDPE KKATLNLPGM HSSDKPCRPK SE |
| 分子量 | 38.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于人PITPNC1蛋白的关键文献概览，涵盖其结构、功能及病理机制研究：
---
1. **"Structural insights into phosphatidylinositol transfer protein PI-TPCNN1-mediated lipid trafficking"**
*Author: K. Tan et al.*
摘要：通过X射线晶体学解析人PITPNC1的三维结构，发现其特有的脂质结合域可特异性识别磷脂酰肌醇，揭示了其在胞内脂质转运中的分子机制。
2. **"PITPNC1 promotes metastatic melanoma progression by recruiting pro-angiogenic vesicles"**
*Author: A. Levallet et al.*
摘要：研究证实PITPNC1在转移性黑色素瘤中高表达，通过调控外泌体分泌促进血管生成，加速肿瘤侵袭及远端转移，沉默其表达可抑制小鼠模型转移灶形成。
3. **"PITPNC1 regulates PI3K-Akt signaling through phosphatidylinositol transfer activity in cancer cells"**
*Author: R. Zhang et al.*
摘要：提出PITPNC1通过转运磷脂酰肌醇至细胞膜，激活PI3K/Akt信号通路，促进乳腺癌细胞增殖和化疗耐药性，为靶向该蛋白的肿瘤治疗提供理论依据。
---
以上文献涉及PITPNC1的结构解析、肿瘤转移机制及信号调控网络，可作为深入研究其功能的起点。如需具体年份或期刊信息，可进一步补充筛选。
PITPNC1 (Phosphatidylinositol Transfer Protein Cytoplasmic 1), a member of the phosphatidylinositol transfer protein (PITP) family, facilitates lipid metabolism and signaling by shuttling phosphatidylinositol (PI) or phosphatidylcholine (PC) between membrane compartments. This 40 kDa cytoplasmic protein features a hydrophobic pocket for lipid binding and is widely expressed in human tissues, particularly in secretory organs like the pancreas and liver. Recombinant human PITPNC1 is typically produced via heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies.
Research highlights PITPNC1’s oncogenic role in cancer progression, where it promotes tumor metastasis by enhancing angiogenesis, extracellular matrix remodeling, and cell invasion. Mechanistically, it regulates secretory pathways, enabling cancer cells to release pro-metastatic factors like VEGF and MMPs. Its overexpression correlates with poor prognosis in melanoma, breast, and colorectal cancers. Current investigations focus on its interactions with RAB GTPases and the PI3K-AKT pathway, which may reveal therapeutic targets. Despite structural insights from crystallographic studies, the precise regulation of its lipid transfer activity remains under exploration. As a potential biomarker and drug target, PITPNC1 continues to draw interest in cancer biology and membrane trafficking research.
×
