| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOTCH4 |
| Uniprot No | Q99466 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NOTCH4重组蛋白的3-4条参考文献示例(注：文献标题与作者为虚构，仅用于示例)：
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1. **标题**：*Expression and Functional Analysis of Recombinant NOTCH4 in Angiogenesis*
**作者**：Smith J, et al.
**摘要**：研究通过哺乳动物细胞系统成功表达功能性NOTCH4重组蛋白，证实其通过激活HES1信号通路促进血管内皮细胞迁移，为血管生成相关疾病提供分子机制依据。
2. **标题**：*Structural Characterization of the NOTCH4 Extracellular Domain Recombinant Protein*
**作者**：Zhang L, et al.
**摘要**：利用昆虫细胞表达并纯化NOTCH4胞外域重组蛋白，通过冷冻电镜解析其三维结构，揭示其与配体结合的特异性构象变化。
3. **标题**：*Recombinant NOTCH4 Enhances Tumor Cell Invasion via EGFR Crosstalk*
**作者**：Brown K, et al.
**摘要**：研究发现重组NOTCH4蛋白在乳腺癌细胞中通过调控EGFR磷酸化促进侵袭，提示NOTCH4-EGFR信号轴可作为癌症治疗靶点。
4. **标题**：*High-Yield Production of NOTCH4 Recombinant Protein for Drug Screening*
**作者**：Wang Y, et al.
**摘要**：开发了一种基于CHO细胞的高效表达体系，规模化生产活性NOTCH4重组蛋白，并应用于小分子抑制剂筛选，发现多个潜在先导化合物。
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**说明**：以上示例聚焦于NOTCH4重组蛋白的表达技术、结构解析、功能机制及药物开发应用，涵盖不同研究方向。实际文献需通过学术数据库(如PubMed、Web of Science)检索关键词“NOTCH4 recombinant protein”获取。
**Background of NOTCH4 Recombinant Protein**
The NOTCH4 protein is a member of the Notch receptor family, a highly conserved group of transmembrane signaling proteins critical for cell-cell communication. Notch signaling regulates diverse biological processes, including cell differentiation, proliferation, and apoptosis, playing essential roles in embryonic development and tissue homeostasis. NOTCH4. encoded by the *NOTCH4* gene in humans, shares structural features with other Notch receptors, including extracellular epidermal growth factor (EGF)-like repeats, a transmembrane domain, and an intracellular region containing signaling domains. Unlike other Notch receptors (NOTCH1-3), NOTCH4 is primarily expressed in vascular endothelial cells and certain immune cells, suggesting specialized roles in angiogenesis and inflammatory responses.
Recombinant NOTCH4 protein is engineered in vitro to study its structure, function, and interactions. It is typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, such as glycosylation, which are vital for ligand binding and receptor activation. The purified protein often includes functional domains like the extracellular ligand-binding region or the intracellular transcriptional regulatory region, depending on research goals.
NOTCH4 dysregulation has been linked to diseases such as cancer, cardiovascular disorders, and immune dysfunctions. For example, aberrant NOTCH4 signaling is implicated in tumor angiogenesis, metastasis, and resistance to therapy. Recombinant NOTCH4 serves as a tool to investigate these mechanisms, screen potential inhibitors, or develop targeted therapies. Additionally, it aids in studying crosstalk between Notch pathways and other signaling cascades (e.g., Wnt or TGF-β).
In summary, NOTCH4 recombinant protein is a pivotal resource for unraveling the biological and pathological roles of NOTCH4. offering insights into therapeutic strategies for diseases tied to Notch signaling aberrations.
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