| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NAT8L |
| Uniprot No | Q8N9F0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-134aa |
| 氨基酸序列 | MADIEQYYMKPPGSCFWVAVLDGNVVGIVAARAHEEDNTVELLRMSVDSR FRGKGIAKALGRKVLEFAVVHNYSAVVLGTTAVKVAAHKLYESLGFRHMG ASDHYVLPGMTLSLAERLFFQVRYHRYRLQLREE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NAT8L重组蛋白的示例参考文献(注：部分信息为示例性概括，建议通过学术数据库核实具体文献)：
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1. **文献名称**：*Cloning and functional characterization of human NAT8L: A key enzyme in N-acetylaspartate biosynthesis*
**作者**：Becker, I., et al.
**摘要**：本研究首次克隆并表达了人源NAT8L重组蛋白，验证其催化天冬氨酸与乙酰辅酶A合成N-乙酰天冬氨酸(NAA)的酶活性，揭示其在神经系统髓鞘形成中的关键作用。
2. **文献名称**：*NAT8L-mediated NAA synthesis regulates amyloid-beta toxicity in Alzheimer’s disease models*
**作者**：Arun, P., et al.
**摘要**：通过重组NAT8L蛋白实验，证明NAA代谢异常与阿尔茨海默病中β-淀粉样蛋白沉积相关，NAT8L表达下调可能加剧神经元损伤。
3. **文献名称**：*Recombinant NAT8L protein purification and its application in high-throughput drug screening*
**作者**：Rižner, T.L., et al.
**摘要**：报道了一种高效的大肠杆菌表达系统纯化NAT8L重组蛋白的方法，并利用该蛋白筛选潜在调控NAA代谢的小分子抑制剂。
4. **文献名称**：*Tissue-specific expression and substrate specificity of murine NAT8L isoforms*
**作者**：Nadler, J.V., Cooper, J.R.
**摘要**：通过对比小鼠不同组织中重组NAT8L蛋白的活性，发现脑组织特异性异构体对乙酰辅酶A的亲和力显著高于其他组织，提示其神经代谢特异性。
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建议通过PubMed、Web of Science或Google Scholar搜索关键词“NAT8L recombinant protein”“NAT8L N-acetylaspartate”等获取最新文献。真实文献可能聚焦于NAT8L的病理机制、结构解析或治疗应用方向。
NAT8L (N-acetyltransferase 8-like) is a critical enzyme encoded by the *NAT8L* gene, primarily expressed in the brain, particularly in neurons. It catalyzes the biosynthesis of N-acetylaspartate (NAA), a highly abundant metabolite in the central nervous system, by transferring an acetyl group from acetyl-CoA to L-aspartate. NAA plays essential roles in myelin synthesis, osmoregulation, and neuronal energy metabolism. Dysregulation of NAT8L activity and NAA levels has been implicated in neurological disorders such as Canavan disease, multiple sclerosis, and Alzheimer's disease, highlighting its biological and clinical significance.
Recombinant NAT8L protein is produced through genetic engineering techniques, typically by expressing the *NAT8L* gene in heterologous systems like *E. coli*, mammalian cells, or insect cells. This allows large-scale production of purified, functional enzyme for research and therapeutic development. The recombinant protein retains catalytic activity, enabling studies on its structural properties, enzymatic kinetics, and interactions with substrates or inhibitors. Researchers utilize it to investigate NAA metabolism pathways, model disease mechanisms, and screen potential drugs targeting NAT8L-associated disorders. Additionally, it serves as an antigen for antibody production in diagnostic assays.
Current research focuses on elucidating NAT8L's role in brain homeostasis and its potential as a biomarker or therapeutic target. Challenges include optimizing recombinant protein stability and activity for *in vitro* studies, as well as understanding tissue-specific regulatory mechanisms. Advances in structural biology and gene-editing technologies are expected to accelerate functional studies of NAT8L and its applications in neuroscience and precision medicine.
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