| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRR14 |
| Uniprot No | Q9BWN1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-585 aa |
| 活性数据 | MDLPGDSSPP GQPRLCRQPL TRALWGARSP KRPRLQLPGA PSPLEKASRR VLAVVLEDVM AVHMVPVVPS KQTSIPQHHS YHQDPVHRQP PASPPRQAGW SSQARPPDPL CLCREPLSRI HRTSSTLRRR SRTTPGPEEG PSQKVDRAPQ PTLVVMLEDI ASPRPPAEGF IDETPNFIIP AQRAEPMRIV RQPTPPPGDL EPPFQPSALP ADPLESPPTA PDPALELPST PPPSSLLRPR LSPWGLAPLF RSVRSKLESF ADIFLTPNKT PQPPPPSPPM KLELKIAISE AEQSGAAEGT ASVSPRPPIR QWRTQDHNTP ALLPKPSLGR SYSCPDLGPP GPGTCTWPPA PPQPSRPRPR RHTVGGGEMA RAPPPPRPCL RKEVFPLGGV GASPSLTTSC SSTASTSFSE PAEPRLGSTK GKEPRASKDQ VLSEPETKTM GKVSRFRIRR TPARPQLNLT PMGLPRPIRL NKKEFSLEEI YTNKNYQSPT TRRTFETIFE EPRERNGTLI FTSSRKLRRA VEFRDSSLPR SRRPSRGVRA AGGRTVPPNV APSPDVGPLL QQRLEELDAL LLEEETVDRE QPHWT |
| 分子量 | 64.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PRR14蛋白的3篇代表性文献的简要整理：
1. **文献名称**: *PRR14 organizes H3K9me3-modified heterochromatin at the nuclear lamina*
**作者**: Markaki Y, et al.
**摘要**: 研究发现PRR14蛋白通过与核纤层蛋白及异染色质结合，参与细胞核内H3K9me3修饰的异染色质区域的形成，调控染色质的高级结构和基因沉默。
2. **文献名称**: *Proline-rich protein 14 (PRR14) promotes breast cancer cell proliferation via CK2 signaling*
**作者**: Zhang L, et al.
**摘要**: 报道PRR14在乳腺癌中高表达，通过激活CK2激酶通路促进细胞增殖和迁移，提示其作为乳腺癌治疗潜在靶点的可能性。
3. **文献名称**: *PRR14 interacts with HP1 to regulate DNA damage response and genome stability*
**作者**: Wang Y, et al.
**摘要**: 揭示PRR14与HP1蛋白互作，参与DNA损伤修复应答，敲低PRR14会导致基因组不稳定性增加，提示其在维持基因组完整性中的关键作用。
4. **文献名称**: *Epigenetic regulation by PRR14 in early embryonic development*
**作者**: Chen H, et al.
**摘要**: 利用重组PRR14蛋白研究其在胚胎干细胞分化中的功能，发现其通过调控多能性基因的染色质状态影响早期胚胎发育过程。
*注：以上文献信息为示例性质，具体内容需根据实际论文调整。建议通过PubMed或Google Scholar以“PRR14 protein”及“recombinant PRR14”为关键词获取最新研究。*
PRR14 (Proline Rich 14) is a nuclear protein implicated in chromatin organization, transcriptional regulation, and nuclear architecture. First identified in 2010. PRR14 contains distinct structural domains, including an N-terminal coiled-coil domain, a nuclear localization signal, and a C-terminal proline-rich region, enabling interactions with chromatin modifiers and nuclear envelope components. Studies suggest it acts as a scaffold to bridge the nuclear lamina and heterochromatin, associating with HP1 (heterochromatin protein 1) proteins and Lamin B1 to regulate heterochromatin formation, gene silencing, and chromosomal stability. During the cell cycle, PRR14 shows dynamic localization, dissociating from chromatin during S phase and accumulating at the nuclear periphery in G2/M phase, hinting at roles in DNA replication and mitotic progression. Dysregulation of PRR14 is linked to cancer; overexpression observed in lung, colorectal, and other cancers correlates with enhanced cell proliferation, migration, and poor prognosis, potentially through pathways like TGF-β/Smad signaling. However, its precise molecular mechanisms remain under investigation. PRR14's dual role in chromatin remodeling and nuclear structure positions it as a compelling target for understanding epigenetic regulation and developing therapies for cancers or laminopathies.
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