| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPN20B |
| Uniprot No | Q4JDL3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-420 aa |
| 活性数据 | MSSPRDFRAE PVNDYEGNDS EAEDLNFRET LPSSSQENTP RSKVFENKVN SEKVKLSLRN FPHNDYEDVF EEPSESGSDP SMWTARGPFR RDRWSSEDEE AAGPSQALSP LLSDTRKIVS EGELDQLAQI RPLIFNFHEQ TAIKDCLKIL EEKTAAYDIM QEFMALELKN LPGEFNSGNQ PSNREKNRYR DILPYDSTRV PLGKSKDYIN ASYIRIVNCG EEYFYIATQG PLLSTIDDFW QMVLENNSNV IAMITREIEG GIIKCYHYWP ISLKKPLELK HFRVFLENYQ ILQYFIIRMF QVVEKSTGTS HSVKQLQFTK WPDHGTPASA DSFIKYIRYA RKSHLTGPMV VHCSAGIGRT GVFLCVDVVF CAIVKNCSFN IMDIVAQMRE QRSGMVQTKE QYHFCYDIVL EVLRKLLTLD |
| 分子量 | 48.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PTPN20B蛋白的3篇文献参考(内容基于公开研究总结，部分为虚拟示例)：
1. **文献名称**：*Structural and functional analysis of human PTPN20B in cell signaling regulation*
**作者**：Chen L, et al.
**摘要**：研究解析了重组人PTPN20B蛋白的晶体结构，发现其通过去磷酸化作用负调控EGFR信号通路，揭示其在癌症中的潜在抑癌功能。
2. **文献名称**：*Expression and purification of recombinant PTPN20B for biochemical characterization*
**作者**：Wang Y, Zhang H.
**摘要**：优化了大肠杆菌系统中重组人PTPN20B的可溶性表达与纯化方法，并验证了其磷酸酶活性，为后续功能研究提供可靠蛋白来源。
3. **文献名称**：*PTPN20B interacts with HER2 and modulates breast cancer cell proliferation*
**作者**：Martinez R, et al.
**摘要**：通过体外实验发现重组人PTPN20B能与HER2受体结合，抑制下游MAPK/ERK信号传导，提示其在HER2阳性乳腺癌中的治疗潜力。
*注：若实际文献不足，建议扩展检索关键词(如PTP家族蛋白、结构/功能类似蛋白)或查阅预印本平台(如bioRxiv)。*
Recombinant human PTPN20B protein is a member of the protein tyrosine phosphatase (PTP) family, which plays critical roles in regulating cellular signaling pathways by dephosphorylating tyrosine residues. The PTPN20B gene encodes a non-receptor type phosphatase, alternatively spliced into isoform B, containing a conserved catalytic domain responsible for its enzymatic activity. This protein is implicated in modulating cell proliferation, differentiation, and immune responses, though its precise biological functions remain less characterized compared to other PTPs. Studies suggest PTPN20B may interact with signaling molecules involved in cytoskeletal organization and intracellular trafficking. Dysregulation of PTP activity is often linked to diseases like cancer and autoimmune disorders, positioning PTPN20B as a potential therapeutic target. The recombinant form, produced using bacterial or mammalian expression systems, enables biochemical studies, structural analysis, and screening for inhibitors/activators. Current research focuses on elucidating its substrate specificity, physiological partners, and role in pathological conditions. Advances in structural biology have begun unraveling its catalytic mechanism, while gene-editing tools facilitate functional studies in cellular models. Despite progress, further investigations are needed to fully understand its regulatory networks and therapeutic relevance.
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