| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PUNC |
| Uniprot No | Q8IVU1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-439 aa |
| 活性数据 | MECCRRATPGTLLLFLAFLLLSSRTARSEEDRDGLWDAWGPWSECSRTCGGGASYSLRRCLSSKSCEGRNIRYRTCSNVDCPPEAGDFRAQQCSAHNDVKHHGQFYEWLPVSNDPDNPCSLKCQAKGTTLVVELAPKVLDGTRCYTESLDMCISGLCQIVGCDHQLGSTVKEDNCGVCNGDGSTCRLVRGQYKSQLSATKSDDTVVAIPYGSRHIRLVLKGPDHLYLETKTLQGTKGENSLSSTGTFLVDNSSVDFQKFPDKEILRMAGPLTADFIVKIRNSGSADSTVQFIFYQPIIHRWRETDFFPCSATCGGGYQLTSAECYDLRSNRVVADQYCHYYPENIKPKPKLQECNLDPCPARWEATPWTACSSSCGGGIQSRAVSCVEEDIQGHVTSVEEWKCMYTPKMPIAQPCNIFDCPKWLAQEWSPVTVPSFFVH |
| 分子量 | 75.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人PUNC蛋白的模拟参考文献(注：PUNC蛋白名称可能与实际研究名称不符，可根据实际需求替换)：
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1. **文献名称**: *Expression and functional characterization of recombinant human PUNC protein in E. coli*
**作者**: Zhang L., et al.
**摘要**: 本研究利用大肠杆菌表达系统成功实现了重组人PUNC蛋白的高效可溶性表达，并通过Ni柱层析纯化获得高纯度蛋白。体外实验表明，PUNC蛋白可通过调控Wnt/β-catenin信号通路抑制肿瘤细胞迁移，为癌症治疗提供潜在靶点。
2. **文献名称**: *Structural insights into PUNC protein's role in neuronal development*
**作者**: Thompson R.K., Singh M.
**摘要**: 通过冷冻电镜解析重组人PUNC蛋白的晶体结构(分辨率2.8 Å)，揭示其C端结构域与神经生长因子受体TrkA的特异性结合机制，证实PUNC在神经元突触形成中的调控作用，为神经系统疾病研究提供结构基础。
3. **文献名称**: *PUNC protein attenuates inflammation via TLR4/NF-κB pathway inhibition*
**作者**: Chen X., et al.
**摘要**: 在CHO细胞中表达糖基化重组人PUNC蛋白，发现其可通过竞争性结合TLR4胞外域，抑制LPS诱导的NF-κB活化及IL-6、TNF-α释放，表明PUNC在脓毒症等炎症性疾病中的治疗潜力。
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如需进一步扩展，可补充蛋白药效学或临床研究文献(如：*In vivo therapeutic efficacy of PEGylated PUNC protein in rheumatoid arthritis models*)。建议通过PubMed/Google Scholar以“recombinant human PUNC protein”、“PUNC function”等关键词检索真实文献。
Recombinant human PUNC (Putative Neuronal Cell adhesion molecule) protein is a genetically engineered biomolecule designed to study neural development and synaptic function. Initially identified through homology with known adhesion molecules, PUNC is believed to play a role in cell-cell interactions, axon guidance, and neural circuit formation. Its structure features extracellular immunoglobulin (Ig) domains and fibronectin type III repeats, typical of cell adhesion proteins involved in neuronal connectivity.
The production of recombinant PUNC involves cloning its coding sequence into expression vectors, commonly using mammalian (e.g., HEK293) or insect cell systems to ensure proper post-translational modifications. This allows researchers to obtain soluble, functional protein for experimental applications. Recombinant PUNC has become a key tool in neuroscience, enabling studies on its role in neurite outgrowth, synapse maturation, and neurological disorders like autism spectrum disorders or schizophrenia, where synaptic adhesion pathways are often disrupted.
Additionally, it serves as a reagent for antibody development, structural analysis (e.g., X-ray crystallography), and high-throughput drug screening targeting adhesion-related pathologies. Recent work also explores its potential as a biomarker in neurodegenerative diseases. The creation of recombinant forms addresses limitations of native protein isolation, providing standardized, scalable material for both basic research and therapeutic exploration.
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