| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PURG |
| Uniprot No | Q9UJV8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-347 aa |
| 活性数据 | MERARRRGGG GGRGRGGKNV GGSGLSKSRL YPQAQHSHYP HYAASATPNQ AGGAAEIQEL ASKRVDIQKK RFYLDVKQSS RGRFLKIAEV WIGRGRQDNI RKSKLTLSLS VAAELKDCLG DFIEHYAHLG LKGHRQEHGH SKEQGSRRRQ KHSAPSPPVS VGSEEHPHSV LKTDYIERDN RKYYLDLKEN QRGRFLRIRQ TMMRGTGMIG YFGHSLGQEQ TIVLPAQGMI EFRDALVQLI EDYGEGDIEE RRGGDDDPLE LPEGTSFRVD NKRFYFDVGS NKYGIFLKVS EVRPPYRNTI TVPFKAWTRF GENFIKYEEE MRKICNSHKE KRMDGRKASG EEQECLD |
| 分子量 | 39.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PURG(Purine-rich element-binding protein gamma)蛋白的示例参考文献(虚构内容，供参考格式)：
1. **文献名称**：*Expression and Functional Analysis of Recombinant Human PURG in Transcriptional Regulation*
**作者**：Smith A, et al.
**摘要**：研究在大肠杆菌中表达重组人PURG蛋白，并验证其通过结合富嘌呤DNA序列调控靶基因转录的活性。发现PURG与RNA聚合酶II相互作用，促进特定启动子活性。
2. **文献名称**：*Structural Insights into PURG-DNA Binding via Cryo-EM*
**作者**：Chen L, et al.
**摘要**：通过冷冻电镜解析重组人PURG蛋白与DNA复合物的结构，揭示了其N端结构域对嘌呤富集序列的特异性识别机制，为靶向PURG的药物设计提供依据。
3. **文献名称**：*Role of Recombinant PURG in Cellular Stress Response*
**作者**：Wang Y, et al.
**摘要**：在HEK293细胞中过表达重组PURG蛋白，发现其通过调控抗氧化反应元件(ARE)减轻氧化应激损伤，提示PURG在细胞应激通路中的潜在功能。
4. **文献名称**：*Purification and Biophysical Characterization of Recombinant Human PURG*
**作者**：Johnson R, et al.
**摘要**：优化重组PURG蛋白的昆虫细胞表达系统，并利用圆二色光谱分析其二级结构，证实其α-螺旋结构对DNA结合活性的重要性。
**注**：以上为模拟示例，实际文献请通过PubMed、Google Scholar或Web of Science检索关键词(如“PURG protein recombinant”或“human PURG function”)。
**Background of Recombinant Human PURG Protein**
Recombinant human PURG protein is a synthesized version of the PURG (purine-rich element-binding protein G) encoded by the *PURG* gene. This protein belongs to the PUR (purine-rich element-binding) family, which regulates transcription and translation by binding to purine-rich sequences in DNA and RNA. PURG plays critical roles in cellular processes, including DNA replication, repair, and RNA metabolism, and is implicated in cell cycle regulation, differentiation, and stress responses. Its ability to interact with nucleic acids and proteins highlights its function as a multifunctional adaptor in genomic stability and gene expression control.
Produced via recombinant DNA technology, the protein is expressed in systems like *E. coli* or mammalian cells, ensuring high purity and bioactivity for research. Studying recombinant PURG aids in deciphering its molecular mechanisms, such as its involvement in chromatin remodeling or neurodegenerative diseases like ALS, where PUR family proteins are linked to RNA processing defects. Additionally, PURG's role in cancer is under exploration due to its potential impact on oncogene regulation. By enabling targeted *in vitro* and *in vivo* studies, recombinant PURG serves as a vital tool for therapeutic discovery and understanding disease pathways.
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