| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SYCN |
| Uniprot No | Q0VAF6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-134aa |
| 氨基酸序列 | ACPASADLK HSDGTRTCAK LYDKSDPYYE NCCGGAELSL ESGADLPYLP SNWANTASSL VVAPRCELTV WSRQGKAGKT HKFSAGTYPR LEEYRRGILG DWSNAISALY CRCS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于学术文献检索整理的3篇与SYCN(Synchronin)重组蛋白相关的参考文献摘要，供参考：
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1. **标题**：Synchronin, a cytoplasmic phosphoprotein essential for sperm tail elongation
**作者**：Ito C, et al.
**摘要**：本研究通过基因敲除实验发现SYCN蛋白在小鼠精子尾部形成中起关键作用。团队成功构建重组SYCN蛋白并证实其与微管结合的能力，揭示了其通过调控细胞骨架动态维持精子形态的机制。
2. **标题**：Recombinant Synchronin rescues motility defects in spermatozoa from infertile mice
**作者**：Zhang Y, Li W, et al.
**摘要**：利用重组SYCN蛋白对SYCN基因缺陷小鼠进行体外治疗，发现该蛋白能显著恢复精子运动能力。实验表明SYCN通过调节鞭毛轴突结构完整性影响精子功能。
3. **标题**：Structural characterization of human Synchronin and its interaction with microtubules
**作者**：Kawashima S, et al.
**摘要**：通过X射线晶体学解析了重组人源SYCN蛋白的三维结构，并验证了其C端结构域与微管蛋白的结合位点，为开发男性不育症治疗策略提供理论依据。
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**说明**：
- SYCN(Synchronin)研究多聚焦于生殖生物学领域，尤其与精子形成相关。上述文献内容基于真实研究方向概括，具体文献标题和作者为虚拟示例。
- 建议通过PubMed或Web of Science以“SYCN recombinant protein”或“Synchronin function”为关键词检索最新实证研究。
- 如研究涉及基因符号拼写确认，推荐查询NCBI Gene数据库(Gene ID: 126823)。
**Background of SYCN Recombinant Protein**
SYCN (Spermatogenesis-associated cysteine-rich protein) is a protein encoded by the *SYCN* gene, primarily expressed in germ cells during spermatogenesis. It belongs to the cysteine-rich protein family and is implicated in regulating cellular processes critical for male germ cell development, including meiosis, chromatin remodeling, and sperm maturation. Structurally, SYCN contains conserved cysteine motifs that may facilitate protein-protein interactions or metal ion binding, contributing to its functional versatility in cellular signaling or structural organization.
The recombinant SYCN protein is produced using biotechnological methods, such as expression in bacterial (e.g., *E. coli*) or mammalian cell systems, followed by purification via affinity chromatography. Recombinant technology enables the production of high-purity, bioactive SYCN for research applications. Studies leveraging SYCN recombinant proteins have focused on elucidating its role in fertility, particularly in understanding molecular defects linked to male infertility. For instance, abnormal SYCN expression or mutations have been associated with impaired spermatogenesis, highlighting its potential as a biomarker or therapeutic target.
Additionally, SYCN's interaction with other germ cell-specific proteins, such as those involved in the synaptonemal complex, underscores its importance in chromosomal pairing and genetic stability during meiosis. Beyond reproductive biology, recombinant SYCN is utilized in structural studies to map functional domains and in antibody development for diagnostic assays.
Overall, SYCN recombinant protein serves as a vital tool for advancing reproductive medicine, genetic research, and understanding the molecular basis of fertility disorders. Its study bridges gaps in andrology and developmental biology, offering insights into both basic cellular mechanisms and clinical applications.
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