| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RCBTB1 |
| Uniprot No | Q8NDN9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-531 aa |
| 活性数据 | MVDVGKWPIF TLLSPQEIAS IRKACVFGTS ASEALYVTDN DEVFVFGLNY SNCLGTGDNQ STLVPKKLEG LCGKKIKSLS YGSGPHVLLS TEDGVVYAWG HNGYSQLGNG TTNQGIAPVQ VCTNLLIKQV VEVACGSHHS MALAADGEVF AWGYNNCGQV GSGSTANQPT PRKVTNCLHI KRVVGIACGQ TSSMAVLDNG EVYGWGYNGN GQLGLGNNGN QLTPVRVAAL HSVCVNQIVC GYAHTLALTD EGLLYAWGAN TYGQLGTGNK NNLLSPAHIM VEKERVVEIA ACHSAHTSAA KTQGGHVYMW GQCRGQSVIL PHLTHFSCTD DVFACFATPA VSWRLLSVEH EDFLTVAESL KKEFDSPETA DLKFRIDGKY IHVHKAVLKI RCEHFRSMFQ SYWNEDMKEV IEIDQFSYPV YRAFLQYLYT DTVDLPPEDA IGLLDLATSY CENRLKKLCQ HIIKRGITVE NAFSLFSAAV RYDAEDLEEF CFKFCINHLT EVTQTAAFWQ MDGPLLKEFI AKASKCGAFK N |
| 分子量 | 58.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RCBTB1蛋白的3篇示例参考文献，包括文献名称、作者及摘要概述：
1. **"RCBTB1 mediates cisplatin resistance in ovarian cancer via regulation of homologous recombination repair"**
- **作者**: Chen et al.
- **摘要**: 研究揭示了重组人RCBTB1蛋白通过调控同源重组修复通路增强卵巢癌细胞对顺铂的耐药性，其高表达与患者预后不良相关。
2. **"Crystal structure of the RCBTB1-Cullin3 complex reveals a novel ubiquitin ligase assembly mechanism"**
- **作者**: Müller et al.
- **摘要**: 利用重组RCBTB1蛋白解析其与Cullin3的复合物晶体结构，阐明了RCBTB1在泛素连接酶复合体中的关键作用，为靶向治疗提供结构依据。
3. **"Recombinant RCBTB1 promotes angiogenesis by modulating VEGF signaling in endothelial cells"**
- **作者**: Kim et al.
- **摘要**: 通过哺乳动物系统表达重组RCBTB1蛋白，证实其通过激活VEGF信号通路促进血管生成，提示其在肿瘤微环境中的潜在调控功能。
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**注**：以上文献为示例性质，实际引用时需检索权威数据库(如PubMed、Web of Science)获取真实发表的研究。建议使用关键词“RCBTB1 recombinant protein”“RCBTB1 functional study”进行深入检索。
Recombinant human RCBTB1 (Regulator of Chromosome Condensation and BTB Domain Containing Protein 1) is a multifunctional protein encoded by the RCBTB1 gene located on chromosome 13q14.3. Structurally, it contains an N-terminal RCC1 (Regulator of Chromosome Condensation) domain, known for chromatin binding and GTPase activation, and a C-terminal BTB (Broad-Complex, Tramtrack, and Bric-a-brac) domain implicated in protein-protein interactions. These domains enable RCBTB1 to participate in diverse cellular processes, including cell cycle regulation, DNA damage response, and intracellular trafficking.
Studies suggest RCBTB1 interacts with RAN-GTPases to influence nucleocytoplasmic transport and mitotic spindle assembly. It also acts as a scaffold protein, modulating transcriptional activity through interactions with chromatin modifiers and transcription factors. Emerging evidence links RCBTB1 dysfunction to pathological conditions. Its genomic locus is frequently deleted in cancers like breast and ovarian tumors, indicating potential tumor suppressor roles. Additionally, RCBTB1 mutations are associated with inherited retinal degeneration, highlighting its importance in retinal cell survival.
Recombinant RCBTB1 is widely used to investigate molecular mechanisms in disease models and to explore therapeutic targets. Its conserved domains and functional versatility make it a key subject in studies bridging cell biology, oncology, and genetic disorders. Further research is needed to fully elucidate its signaling networks and clinical relevance.
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