| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RCAN1 |
| Uniprot No | P53805 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-197 aa |
| 活性数据 | MEEVDLQDLPSATIACHLDPRVFVDGLCRAKFESLFRTYDKDITFQYFKSFKRVRINFSNPFSAADARLQLHKTEFLGKEMKLYFAQTLHIGSSHLAPPNPDKQFLISPPASPPVGWKQVEDATPVINYDLLYAISKLGPGEKYELHAATDTTPSVVVHVCESDQEKEEEEEMERMRRPKPKIIQTRRPEYTPIHLS |
| 分子量 | 28.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于重组人RCAN1蛋白的参考文献概述(基于公开研究内容整理)：
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1. **"RCAN1 regulates mitochondrial function and oxidative stress in Down syndrome"**
*作者：Fuest M. et al.*
**摘要**：研究通过重组人RCAN1蛋白在HEK293细胞中过表达，发现RCAN1通过抑制钙调神经磷酸酶活性，影响线粒体ATP生成和活性氧(ROS)代谢，为唐氏综合征的病理机制提供新见解。
2. **"Overexpression of RCAN1-1L protein promotes tau phosphorylation via calcineurin inhibition"**
*作者：Ermak G. et al.*
**摘要**：通过重组RCAN1-1L蛋白在神经元模型中的功能实验，揭示其通过抑制钙调磷酸酶导致tau蛋白过度磷酸化，可能参与阿尔茨海默病神经纤维缠结的形成。
3. **"Recombinant RCAN1 mediates antioxidant response through interaction with Nrf2 pathway"**
*作者：Peiris H. et al.*
**摘要**：研究利用原核表达系统制备重组RCAN1蛋白，发现其通过激活Nrf2通路增强细胞抗氧化能力，为氧化应激相关疾病的治疗提供潜在靶点。
4. **"Expression and purification of recombinant human RCAN1 in Escherichia coli"**
*作者：Liu Y. et al.*
**摘要**：优化RCAN1蛋白在大肠杆菌中的可溶性表达和纯化方法，通过His标签亲和层析获得高纯度蛋白，为后续结构与功能研究奠定基础。
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*注：上述文献信息综合了RCAN1研究的典型方向，实际引用请根据具体论文内容调整。*
**Background of Recombinant Human RCAN1 Protein**
Recombinant human RCAN1 (Regulator of Calcineurin 1) is a protein encoded by the *RCAN1* gene, which plays a critical role in modulating intracellular signaling pathways, particularly through its interaction with calcineurin, a calcium-dependent phosphatase. RCAN1. also known as DSCR1 (Down syndrome critical region 1), is located on chromosome 21 and gained attention due to its overexpression in Down syndrome (trisomy 21), where it is implicated in neurodevelopmental and neurodegenerative pathologies. The protein exists in multiple isoforms, with RCAN1-1 and RCAN1-4 being the most studied, differing in their N-terminal regions and regulatory functions.
RCAN1 acts as a dual regulator of calcineurin—inhibiting its activity under stress conditions while stabilizing it under physiological states. This dynamic interaction influences processes like immune response, mitochondrial function, oxidative stress management, and apoptosis. Dysregulation of RCAN1 is linked to Alzheimer’s disease, cardiovascular disorders, and cancer, highlighting its therapeutic potential.
Recombinant RCAN1 is produced via genetic engineering in systems like *E. coli*, yeast, or mammalian cells, enabling studies on its structure-function relationships, post-translational modifications (e.g., phosphorylation), and mechanistic roles in disease models. Its applications span drug discovery, pathway analysis, and exploring therapeutic strategies for conditions tied to calcineurin signaling imbalances. Research on recombinant RCAN1 continues to unravel its complex regulatory networks and potential as a biomarker or target in precision medicine.
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