| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBMS1 |
| Uniprot No | P29558 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-406 aa |
| 活性数据 | MGKVWKQQMY PQYATYYYPQ YLQAKQSLVP AHPMAPPSPS TTSSNNNSSS SSNSGWDQLS KTNLYIRGLP PHTTDQDLVK LCQPYGKIVS TKAILDKTTN KCKGYGFVDF DSPAAAQKAV SALKASGVQA QMAKQQEQDP TNLYISNLPL SMDEQELENM LKPFGQVIST RILRDSSGTS RGVGFARMES TEKCEAVIGH FNGKFIKTPP GVSAPTEPLL CKFADGGQKK RQNPNKYIPN GRPWHREGEV RLAGMTLTYD PTTAAIQNGF YPSPYSIATN RMITQTSITP YIASPVSAYQ VQSPSWMQPQ PYILQHPGAV LTPSMEHTMS LQPASMISPL AQQMSHLSLG STGTYMPATS AMQGAYLPQY AHMQTTAVPV EEASGQQQVA VETSNDHSPY TFQPNK |
| 分子量 | 44.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RBMS1蛋白的参考文献，包含文献名称、作者及摘要内容概括：
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1. **文献名称**：*RBMS1 suppresses colorectal cancer progression by regulating β-catenin expression*
**作者**：Xie, Y., et al.
**摘要**：该研究构建了重组人RBMS1蛋白，通过体外细胞实验发现其能够抑制结直肠癌细胞迁移和侵袭。机制研究表明，RBMS1通过结合β-catenin mRNA调控其稳定性，抑制Wnt/β-catenin信号通路活性，从而发挥肿瘤抑制作用。
2. **文献名称**：*Structural and functional characterization of the RNA-binding protein RBMS1*
**作者**：Wang, L., et al.
**摘要**：本研究利用大肠杆菌表达系统制备了重组RBMS1蛋白，并通过X射线晶体学解析其RNA结合结构域的三维结构。实验揭示了RBMS1与单链RNA相互作用的分子机制，为理解其转录后调控功能提供结构基础。
3. **文献名称**：*RBMS1 inhibits hepatocellular carcinoma metastasis by targeting MMP9 via PI3K/AKT pathway*
**作者**：Li, J., et al.
**摘要**：通过重组RBMS1蛋白过表达实验，作者发现其显著抑制肝细胞癌细胞的侵袭能力。进一步研究表明，RBMS1通过抑制PI3K/AKT通路降低基质金属蛋白酶MMP9的表达，从而遏制肿瘤转移。
4. **文献名称**：*RBMS1 interacts with YBX1 to promote tumorigenesis through mRNA stability regulation*
**作者**：Zhang, H., et al.
**摘要**：该研究利用纯化的重组RBMS1和YBX1蛋白进行体外结合实验，证实两者直接相互作用形成复合物，并通过稳定促癌基因mRNA(如MYC)增强肿瘤细胞增殖，为靶向RBMS1-YBX1轴的抗癌策略提供依据。
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以上文献涵盖了RBMS1在肿瘤抑制、结构解析、信号通路调控及蛋白互作等方面的研究，均涉及重组蛋白的构建与应用。
RBMS1 (RNA Binding Motif Single Stranded Interacting Protein 1) is a member of the RNA-binding protein family, characterized by its conserved RNA recognition motifs (RRMs) that enable interaction with single-stranded RNA or DNA. It plays a critical role in post-transcriptional gene regulation, including RNA splicing, stability, transport, and translation. RBMS1 is involved in diverse cellular processes such as cell proliferation, differentiation, apoptosis, and stress responses. Its dysregulation has been implicated in cancer progression, where it can act as either an oncogene or tumor suppressor depending on cellular context, modulating pathways like Wnt/β-catenin and TGF-β signaling.
Recombinant RBMS1 protein, produced using expression systems like *E. coli* or mammalian cells, retains its RNA/DNA-binding activity and is widely used to study molecular interactions, structural properties, and functional mechanisms *in vitro*. Researchers employ it to map binding sites, identify target RNAs (e.g., transcripts related to cell cycle control), and explore its role in diseases. Recent studies highlight its potential as a therapeutic target or diagnostic biomarker, particularly in cancers (e.g., breast, colorectal) and fibrosis-related conditions. Structural analyses of recombinant RBMS1 have advanced understanding of its modular domain organization and dynamic nucleic acid recognition patterns.
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