| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBM6 |
| Uniprot No | P78332 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-601 aa |
| 活性数据 | MIQDKEVTLEYVSSLDFWYCKRCKANIGGHRSSCSFCKNPREVTEAKQELITYPQPQKTSIPAPLEKQPNQPLRPADKEPEPRKREEGQESRLGHQKREAERYLPPSRREGPTFRRDRERESWSGETRQDGESKTIMLKRIYRSTPPEVIVEVLEPYVRLTTANVRIIKNRTGPMGHTYGFIDLDSHAEALRVVKILQNLDPPFSIDGKMVAVNLATGKRRNDSGDHSDHMHYYQGKKYFRDRRGGGRNSDWSSDTNRQGQQSSSDCYIYDSATGYYYDPLAGTYYDPNTQQEVYVPQDPGLPEEEEIKEKKPTSQGKSSSKKEMSKRDGKEKKDRGVTRFQENASEGKAPAEDVFKKPLPPTVKKEESPPPPKVVNPLIGLLGEYGGDSDYEEEEEEEQTPPPQPRTAQPQKREEQTKKENEEDKLTDWNKLACLLCRRQFPNKEVLIKHQQLSDLHKQNLEIHRKIKQSEQELAYLERREREGKFKGRGNDRREKLQSFDSPERKRIKYSRETDSDRKLVDKEDIDTSSKGGCVQQATGWRKGTGLGYGHPGLASSEEAEGRMRGPSVGASGRTSKRQSNETYRDAVRRVMFARYKELD |
| 分子量 | 95.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RBM6蛋白研究的3篇示例参考文献(文献为虚构示例，建议通过学术数据库核实实际文献)：
1. **《RBM6 regulates alternative splicing of BCL2L12 and promotes apoptosis in lung cancer cells》**
- **作者**: Smith J, et al. (2020)
- **摘要**: 研究揭示了RBM6通过调控BCL2L12基因的可变剪接，促进促凋亡亚型的表达，抑制肺癌细胞存活，表明其可能作为抑癌因子发挥作用。
2. **《Structural and functional analysis of the RNA-binding motifs in human RBM6 protein》**
- **作者**: Chen L, et al. (2018)
- **摘要**: 解析了重组人RBM6蛋白的RNA结合结构域(RBM)的三维结构，并证明其特异性结合GU富含区域的RNA序列，调控靶基因的剪接活性。
3. **《RBM6 interacts with p53 and modulates DNA damage response in hepatocellular carcinoma》**
- **作者**: Wang Y, et al. (2022)
- **摘要**: 发现RBM6与p53蛋白相互作用，增强DNA损伤后p21的转录激活，抑制肝细胞癌的增殖，提示其作为癌症治疗新靶点的潜力。
**建议**：实际文献可通过PubMed、Google Scholar等平台以"RBM6 protein"、"RNA-binding motif protein 6"为关键词搜索，注意筛选近5年文献以获取最新进展。
Recombinant human RNA-binding motif protein 6 (RBM6) is a multifunctional RNA-binding protein encoded by the *RBM6* gene, located on chromosome 3p21.3. Structurally, RBM6 contains conserved RNA recognition motifs (RRMs) and a prion-like domain, enabling its role in RNA processing, splicing, stability regulation, and transcript transport. It interacts with spliceosome components and other RNA-binding proteins (e.g., RBM5. RBM10) to modulate alternative splicing events linked to cellular homeostasis, apoptosis, and DNA repair. Dysregulation of RBM6 is implicated in cancer pathogenesis, including lung, liver, and colorectal cancers, where it often exhibits reduced expression and may act as a tumor suppressor. Studies highlight its role in regulating transcripts of oncogenes (e.g., *BCL2L1*, *MYC*) and tumor suppressors, influencing cell cycle progression and metastatic potential. Additionally, RBM6 mutations or aberrant splicing isoforms are associated with neurological disorders, such as amyotrophic lateral sclerosis (ALS), suggesting broader roles in neurodegenerative pathways. Recombinant RBM6 is commonly expressed in *E. coli* or mammalian systems for functional studies, aiding mechanistic insights into its RNA-binding specificity and disease-related interactions. Ongoing research explores its therapeutic potential as a biomarker or target for RNA-centric therapies in oncology and neurology.
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