| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBM4 |
| Uniprot No | Q9BWF3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-364 aa |
| 活性数据 | MVKLFIGNLP REATEQEIRS LFEQYGKVLE CDIIKNYGFV HIEDKTAAED AIRNLHHYKL HGVNINVEAS KNKSKTSTKL HVGNISPTCT NKELRAKFEE YGPVIECDIV KDYAFVHMER AEDAVEAIRG LDNTEFQGKR MHVQLSTSRL RTAPGMGDQS GCYRCGKEGH WSKECPIDRS GRVADLTEQY NEQYGAVRTP YTMSYGDSLY YNNAYGALDA YYKRCRAARS YEAVAAAAAS VYNYAEQTLS QLPQVQNTAM ASHLTSTSLD PYDRHLLPTS GAAATAAAAA AAAAAVTAAS TSYYGRDRSP LRRATAPVPT VGEGYGYGHE SELSQASAAA RNSLYDMARY EREQYADRAR YSAF |
| 分子量 | 40.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RBM4蛋白的3篇代表性文献，简要总结如下：
1. **文献名称**：*RBM4 regulates neuronal differentiation of mesenchymal stem cells by modulating alternative splicing of a core transcription factor, WT1*
**作者**：Lin, J.-H., et al.
**摘要**：该研究发现RBM4通过调控转录因子WT1的选择性剪接(促进外显子5的跳跃)，影响间充质干细胞的神经元分化，揭示了RBM4在细胞命运决定中的RNA剪接调控机制。
2. **文献名称**：*Muscleblind-like 1 and RBM4 competitively regulate alternative splicing of CELF1-controlled exons during muscle cell differentiation*
**作者**：Takahashi, Y., et al.
**摘要**：研究揭示了RBM4与肌肉盲样蛋白(MBNL1)在肌肉细胞分化中的拮抗作用，两者通过竞争调控CELF1依赖性外显子的选择性剪接，进而协调肌肉特异性基因表达网络。
3. **文献名称**：*RBM4 suppresses tumor progression by stabilizing PTEN mRNA in lung adenocarcinoma*
**作者**：Chen, Q., et al.
**摘要**：该论文表明，RBM4通过直接结合PTEN mRNA的3'UTR并增强其稳定性，抑制PI3K/AKT信号通路，从而阻碍肺癌细胞的增殖和转移，提示其作为肿瘤抑制因子的潜在机制。
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注：以上文献为虚拟归纳示例，实际研究中请根据具体课题在PubMed或Web of Science等数据库检索最新论文。
**Background of Recombinant Human RBM4 Protein**
Recombinant human RNA-binding motif protein 4 (RBM4) is a multifunctional RNA-binding protein crucial for post-transcriptional gene regulation. Structurally, RBM4 contains two RNA recognition motifs (RRMs) and a C-terminal domain involved in protein interactions, enabling its role in alternative splicing, mRNA stability, and translation. It regulates splicing by binding exonic or intronic regions of target pre-mRNAs, influencing tissue-specific isoform expression, particularly in muscles and the nervous system.
RBM4 is ubiquitously expressed but shows elevated levels in metabolically active tissues like the heart, skeletal muscle, and brain. It plays vital roles in cellular differentiation, embryonic development, and stress responses. For example, RBM4 promotes myogenesis by modulating splicing of transcripts like *Mef2C* and interacts with microRNAs (e.g., miR-20b) to fine-tune gene expression. Dysregulation of RBM4 is linked to diseases, including cancer, where it can act as a tumor suppressor or promoter depending on context, and neurological disorders due to aberrant RNA processing.
The protein undergoes post-translational modifications, such as phosphorylation, which alter its subcellular localization and activity under stress. Two isoforms, RBM4A and RBM4B, encoded by separate genes on chromosomes 11 and 19. exhibit overlapping but distinct functions. Their roles in balancing RNA metabolism highlight RBM4's importance in maintaining cellular homeostasis, making it a potential therapeutic target for splicing-related pathologies. Research continues to unravel its complex regulatory networks and disease implications.
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