| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBM47 |
| Uniprot No | A0AV96 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-593 aa |
| 活性数据 | MTAEDSTAAM SSDSAAGSSA KVPEGVAGAP NEAALLALME RTGYSMVQEN GQRKYGGPPP GWEGPHPQRG CEVFVGKIPR DVYEDELVPV FEAVGRIYEL RLMMDFDGKN RGYAFVMYCH KHEAKRAVRE LNNYEIRPGR LLGVCCSVDN CRLFIGGIPK MKKREEILEE IAKVTEGVLD VIVYASAADK MKNRGFAFVE YESHRAAAMA RRKLMPGRIQ LWGHQIAVDW AEPEIDVDED VMETVKILYV RNLMIETTED TIKKSFGQFN PGCVERVKKI RDYAFVHFTS REDAVHAMNN LNGTELEGSC LEVTLAKPVD KEQYSRYQKA ARGGGAAEAA QQPSYVYSCD PYTLAYYGYP YNALIGPNRD YFVKAGSIRG RGRGAAGNRA PGPRGSYLGG YSAGRGIYSR YHEGKGKQQE KGYELVPNLE IPTVNPVAIK PGTVAIPAIG AQYSMFPAAP APKMIEDGKI HTVEHMISPI AVQPDPASAA AAAAAAAAAA AAVIPTVSTP PPFQGRPITP VYTVAPNVQR IPTAGIYGAS YVPFAAPATA TIATLQKNAA AAAAMYGGYA GYIPQAFPAA AIQVPIPDVY QTY |
| 分子量 | 64.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人(RBM47)蛋白功能相关的3篇文献示例(注：文献标题和作者为虚构示例，实际研究中请核对真实文献)：
1. **文献名称**：*RBM47 suppresses colorectal cancer by destabilizing β-catenin mRNA via direct interaction*
**作者**：Liu Y, et al.
**摘要**：研究发现RBM47通过结合β-catenin mRNA的3'UTR区，促进其降解，抑制Wnt/β-catenin信号通路，从而抑制结直肠癌的增殖和转移。
2. **文献名称**：*RBM47 regulates alternative splicing of tumor-related genes in hepatocellular carcinoma*
**作者**：Wang X, et al.
**摘要**：RBM47通过调控肝癌细胞中促癌基因(如EGFR)的可变剪接，抑制异常剪接异构体的形成，进而延缓肿瘤进展并增强化疗敏感性。
3. **文献名称**：*RBM47 maintains intestinal stem cell homeostasis by stabilizing Lgr5 mRNA*
**作者**：Chen L, et al.
**摘要**：在小肠干细胞中，RBM47通过结合Lgr5 mRNA并维持其稳定性，促进干细胞的自我更新和分化平衡，缺失RBM47导致肠道稳态紊乱。
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如需真实文献，建议在PubMed或Google Scholar搜索关键词“RBM47 function”、“RBM47 cancer”等获取。
**Background of Recombinant Human RBM47 Protein**
Recombinant human RBM47 (RNA Binding Motif Protein 47) is a protein engineered through molecular cloning techniques to mimic the native RBM47 expressed in humans. RBM47 belongs to the RNA-binding protein family, characterized by conserved RNA recognition motifs (RRMs) that facilitate interactions with RNA molecules. This protein plays a critical role in post-transcriptional gene regulation, including mRNA splicing, stability, and translation, thereby influencing cellular processes such as differentiation, apoptosis, and stress responses.
Native RBM47 is implicated in tissue development and homeostasis, with high expression observed in organs like the intestines, liver, and kidneys. Studies highlight its tumor-suppressive functions in certain cancers, where it modulates oncogenic pathways by binding to specific RNA targets or interacting with signaling molecules like β-catenin. Dysregulation of RBM47 has been linked to pathological conditions, including colorectal cancer and metabolic disorders.
The recombinant form of RBM47 is typically produced in *E. coli* or mammalian cell systems, enabling controlled studies of its biochemical properties, structural interactions, and therapeutic potential. Its availability accelerates research into RNA-protein dynamics, disease mechanisms, and the development of RNA-targeted therapies. As a tool, recombinant RBM47 also aids in identifying RNA-binding partners and validating genetic networks influenced by this multifaceted regulator. Ongoing research aims to elucidate its precise molecular roles and exploit its functions in biomedical applications.
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