| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBM35A |
| Uniprot No | Q6NXG1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-681 aa |
| 活性数据 | MTASPDYLVV LFGITAGATG AKLGSDEKEL ILLFWKVVDL ANKKVGQLHE VLVRPDQLEL TEDCKEETKI DVESLSSASQ LDQALRQFNQ SVSNELNIGV GTSFCLCTDG QLHVRQILHP EASKKNVLLP ECFYSFFDLR KEFKKCCPGS PDIDKLDVAT MTEYLNFEKS SSVSRYGASQ VEDMGNIILA MISEPYNHRF SDPERVNYKF ESGTCSKMEL IDDNTVVRAR GLPWQSSDQD IARFFKGLNI AKGGAALCLN AQGRRNGEAL VRFVSEEHRD LALQRHKHHM GTRYIEVYKA TGEDFLKIAG GTSNEVAQFL SKENQVIVRM RGLPFTATAE EVVAFFGQHC PITGGKEGIL FVTYPDGRPT GDAFVLFACE EYAQNALRKH KDLLGKRYIE LFRSTAAEVQ QVLNRFSSAP LIPLPTPPII PVLPQQFVPP TNVRDCIRLR GLPYAATIED ILDFLGEFAT DIRTHGVHMV LNHQGRPSGD AFIQMKSADR AFMAAQKCHK KNMKDRYVEV FQCSAEEMNF VLMGGTLNRN GLSPPPCKLP CLSPPSYTFP APAAVIPTEA AIYQPSVILN PRALQPSTAY YPAGTQLFMN YTAYYPSPPG SPNSLGYFPT AANLSGVPPQ PGTVVRMQGL AYNTGVKEIL NFFQGYQYAT EDGLIHTNDQ ARTLPKEWVC I |
| 分子量 | 75.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是构造的示例参考文献(请注意，"RBM35A"可能并非标准命名，建议核实基因/蛋白名称的正确性，如是否应为RBM35或其他变体)：
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1. **文献名称**: *Role of RBM35A in mRNA Splicing and Cellular Stress Response*
**作者**: Smith J, et al.
**摘要**: 研究报道了重组人RBM35A蛋白作为RNA结合蛋白参与pre-mRNA剪接调控，在氧化应激条件下通过调控靶基因的可变剪接影响细胞存活。
2. **文献名称**: *Recombinant Human RBM35A Expression and Purification for Structural Studies*
**作者**: Tanaka M, et al.
**摘要**: 描述了RBM35A蛋白的重组表达系统(大肠杆菌体系)及纯化方法，并通过结晶实验分析其RNA结合结构域的构象特征。
3. **文献名称**: *RBM35A Dysregulation Promotes Tumor Metastasis via EMT Pathway Activation*
**作者**: Chen X, et al.
**摘要**: 发现RBM35A在癌细胞中异常高表达，通过诱导上皮-间质转化(EMT)促进肿瘤侵袭，提示其作为潜在治疗靶点。
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**注意**：以上文献为示例性内容。若实际检索无果，请检查以下可能性：
1. 目标蛋白是否为**RBM45**(与神经退行性疾病相关)或**RBM25**(参与剪接调控)等近似名称;
2. 是否涉及**RNA-binding motif (RBM)**家族其他成员的功能研究。
建议通过数据库(如UniProt、PubMed)确认准确命名后再进行文献检索。
Recombinant human RBM35A (RNA-binding motif protein 35A), also known as SERPINE1 mRNA-binding protein 1 (SERBP1), is a multifunctional protein involved in RNA metabolism and cellular regulation. It contains conserved RNA-binding domains, including the RG/RGG motif, enabling interactions with mRNA and non-coding RNAs. RBM35A plays roles in post-transcriptional gene regulation, such as mRNA stability, splicing, and translation control. Studies link it to pathways influencing cell proliferation, apoptosis, and stress responses, with implications in cancer, neurodegenerative diseases, and metabolic disorders. Its overexpression is observed in certain cancers, where it may promote tumor growth by modulating oncogenic RNA networks. Recombinant RBM35A is typically produced in *E. coli* or mammalian expression systems, purified via affinity chromatography (e.g., His-tag), and used to study RNA-protein interactions, molecular pathways, or therapeutic targeting. Structural analyses reveal dynamic conformations that adapt to RNA ligands, highlighting its regulatory versatility. Current research focuses on deciphering its precise mechanisms in disease pathogenesis and exploring its potential as a diagnostic biomarker or therapeutic target.
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