| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBM22 |
| Uniprot No | Q9NW64 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-420 aa |
| 活性数据 | ATSLGSNTY NRQNWEDADF PILCQTCLGE NPYIRMTKEK YGKECKICAR PFTVFRWCPG VRMRFKKTEV CQTCSKLKNV CQTCLLDLEY GLPIQVRDAG LSFKDDMPKS DVNKEYYTQN MEREISNSDG TRPVGMLGKA TSTSDMLLKL ARTTPYYKRN RPHICSFWVK GECKRGEECP YRHEKPTDPD DPLADQNIKD RYYGINDPVA DKLLKRASTM PRLDPPEDKT ITTLYVGGLG DTITETDLRN HFYQFGEIRT ITVVQRQQCA FIQFATRQAA EVAAEKSFNK LIVNGRRLNV KWGRSQAARG KEKEKDGTTD SGIKLEPVPG LPGALPPPPA AEEEASANYF NLPPSGPPAV VNIALPPPPG IAPPPPPGFG PHMFHPMGPP PPFMRAPGPI HYPSQDPQRM GAHAGKHSSP |
| 分子量 | 46.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RBM22蛋白的参考文献示例(均为模拟内容，实际引用需查询真实文献)：
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1. **文献名称**：*RBM22 regulates pre-mRNA splicing through direct interaction with the spliceosome*
**作者**：Körner S. et al.
**摘要**：该研究通过生化实验和剪接体复合物分析，揭示了RBM22在剪接体组装中的关键作用，其通过与U5/U6 snRNP相互作用，确保剪接位点的精准识别和催化步骤的完成。
2. **文献名称**：*RBM22 promotes hepatocellular carcinoma progression by modulating mTOR signaling*
**作者**：Li X. et al.
**摘要**：研究发现，RBM22在肝细胞癌中高表达，通过激活mTOR通路促进肿瘤细胞增殖和转移。敲低RBM22显著抑制小鼠模型中肿瘤生长，提示其作为潜在治疗靶点。
3. **文献名称**：*Structural insights into RBM22-RNA interactions during spliceosome remodeling*
**作者**：Gupta A. et al.
**摘要**：利用冷冻电镜解析了RBM22与剪接体RNA结合的复合物结构，揭示其锌指结构域特异性识别pre-mRNA的保守序列，为剪接体动态重组的分子机制提供依据。
4. **文献名称**：*RBM22 deficiency triggers genomic instability via impaired DNA damage response*
**作者**：Wang Y. et al.
**摘要**：该研究证实RBM22缺失导致DNA损伤修复异常，RBM22通过与BRCA1蛋白互作维持基因组稳定性，其功能缺陷与多种癌症的发病风险相关。
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**注意**：以上文献为示例，实际研究中请通过PubMed、Web of Science等平台查询真实发表的论文。
Recombinant human RBM22 (RNA-binding motif protein 22) is a protein encoded by the *RBM22* gene, which plays a critical role in RNA splicing and cellular RNA metabolism. As an RNA-binding protein, RBM22 contains conserved domains, including an RNA recognition motif (RRM) and a zinc finger structure, enabling interactions with spliceosomal components and pre-mRNA. It is essential for the assembly and stabilization of the spliceosome during pre-mRNA processing, particularly in the catalytic activation phase of splicing.
RBM22 also participates in maintaining genomic stability by regulating cell cycle progression and the DNA damage response. Dysregulation of RBM22 has been linked to various diseases, including cancers, where aberrant expression correlates with tumor progression and patient prognosis. For example, it may act as an oncogene in hepatocellular carcinoma or a tumor suppressor in other contexts, highlighting its context-dependent roles.
Recombinant RBM22 is produced using biotechnological systems (e.g., *E. coli* or mammalian cell cultures) for functional studies, enabling researchers to explore its molecular mechanisms in RNA processing, genome integrity, and disease pathways. Its applications extend to *in vitro* assays, structural analyses, and drug discovery targeting splicing-related disorders. Further research aims to clarify its dual roles in cancer and therapeutic potential.
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