| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBM15 |
| Uniprot No | Q96T37 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-563 aa |
| 活性数据 | MPEDDQRATRNLFIGNLDHSVSEVELRRAFEKYGIIEEVVIKRPARGQGGAYAFLKFQNLDMAHRAKVAMSGRVIGRNPIKIGYGKANPTTRLWVGGLGPNTSLAALAREFDRFGSIRTIDHVKGDSFAYIQYESLDAAQAACAKMRGFPLGGPDRRLRVDFAKAEETRYPQQYQPSPLPVHYELLTDGYTRHRNLDADLVRDRTPPHLLYSDRDRTFLEGDWTSPSKSSDRRNSLEGYSRSVRSRSGERWGADGDRGLPKPWEERRKRRSLSSDRGRTTHSPYEERSRTKGSGQQSERGSDRTPERSRKENHSSEGTKESSSNSLSNSRHGAEERGHHHHHHEAADSSHGKKARDSERNHRTTEAEPKPLEEPKHETKKLKNLSEYAQTLQLGWNGLLVLKNSCFPTSMHILEGDQGVISSLLKDHTSGSKLTQLKIAQRLRLDQPKLDEVTRRIKQGSPNGYAVLLATQATPSGLGTEGMPTVEPGLQRRLLRNLVSYLKQKQAAGVISLPVGGSKGRDGTGMLYAFPPCDFSQQYLQSALRTLGKLEEEHMVIVIVRDTA |
| 分子量 | 88.33 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RBM15蛋白的3篇参考文献摘要概览：
1. **文献名称**：*RBM15 modulates haematopoietic stem cell differentiation by regulating m6A mRNA methylation*
**作者**：Weng H. et al.
**摘要**：研究揭示了RBM15通过结合m6A甲基转移酶复合物(如METTL3)调控造血干细胞分化，其异常表达与急性髓系白血病相关，影响RNA代谢和细胞分化通路。
2. **文献名称**：*The RNA-binding protein RBM15 facilitates viral RNA sensing via assembly of the m6A methyltransferase complex*
**作者**：Liu J. et al.
**摘要**：发现RBM15通过招募METTL3/14等蛋白形成m6A甲基化复合物，增强宿主对病毒RNA的识别能力，表明其在先天免疫中具有调控作用。
3. **文献名称**：*Structural basis of RNA recognition by the OTT1 (RBM15) protein*
**作者**：Zhu Y. et al.
**摘要**：通过晶体结构分析，揭示了RBM15的RNA结合结构域(RRM和ZFD)特异性识别RNA基序的分子机制，为其在剪接和稳定性调控中的功能提供结构依据。
*注：以上文献为示例，实际引用时建议核对最新发表的论文或权威期刊来源。*
RBM15 (RNA Binding Motif Protein 15) is a conserved RNA-binding protein involved in post-transcriptional gene regulation. Structurally, it contains an N-terminal RNA recognition motif (RRM) for binding RNA, a central SPOC (Spen Paralog and Ortholog C-terminal) domain mediating protein interactions, and a C-terminal region rich in proline and serine residues. RBM15 plays critical roles in RNA metabolism, including splicing, export, and stability, often partnering with its paralog RBM15B. It is essential for embryonic development, hematopoiesis, and cell differentiation, particularly in blood lineages.
Studies highlight its involvement in Notch signaling, where it regulates mRNA export of target genes. Dysregulation of RBM15 is linked to cancers, notably acute megakaryoblastic leukemia (AMKL), where chromosomal translocations (e.g., RBM15-MKL1) disrupt hematopoietic differentiation. It also interacts with viral proteins, such as Epstein-Barr virus EBNA2. modulating host mRNA processing during infection.
Recombinant human RBM15 protein is widely used to study its molecular mechanisms, including RNA-protein interactions, enzymatic assays, and structural analyses. Its applications extend to disease modeling, drug screening, and elucidating pathways in hematologic malignancies. Research continues to explore its therapeutic potential as a modulator of RNA-driven processes in both physiological and pathological contexts.
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