| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBED1 |
| Uniprot No | Q96FG2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-381 aa |
| 活性数据 | MNEKSCSFHS KEELRDGQGE RLSAGYSPSY DKDKSVLAFR GIPISELKNH GILQALTTEA YEWEPRVVST EVVRAQEEWE AVDTIQPETG SQASSEQPGQ LISFSEALQH FQTVDLSPFK KRIQPTIRRT GLAALRHYLF GPPKLHQRLR EERDLVLTIA QCGLDSQDPV HGRVLQTIYK KLTGSKFDCA LHGNHWEDLG FQGANPATDL RGAGFLALLH LLYLVMDSKT LPMAQEIFRL SRHHIQQFPF CLMSVNITHI AIQALREECL SRECNRQQKV IPVVNSFYAA TFLHLAHVWR TQRKTISDSG FVLKELEVLA KKSPRRLLKT LELYLARVSK GQASLLGAQK CYGPEAPPFK DLTFTGESDL QSHSSEGVWL I |
| 分子量 | 43.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于常见重组蛋白研究模式假设的参考文献示例(文献为虚构示例，仅供参考格式)：
1. **文献名称**: "Recombinant Human RBED1 Protein: Structural Characterization and RNA-Binding Activity"
**作者**: Zhang, Y., et al.
**摘要**: 本研究成功表达并纯化了重组人RBED1蛋白，通过X射线晶体学解析其三维结构，证实其含有典型的RNA识别基序(RRM)。体外实验表明RBED1特异性结合mRNA前体的剪接位点区域，提示其在RNA加工中的作用。
2. **文献名称**: "RBED1 Regulates Ribosome Biogenesis via Interaction with Nucleolar Proteins"
**作者**: Tanaka, K., et al.
**摘要**: 发现RBED1在核仁中富集，与核磷蛋白(NPM1)相互作用，调控rRNA的成熟。敲低RBED1导致核仁结构紊乱及细胞增殖抑制，表明其在核糖体组装中的必要性。
3. **文献名称**: "Dysregulation of RBED1 in Colorectal Cancer and Its Impact on Metastasis"
**作者**: Wang, H., et al.
**摘要**: 分析结直肠癌组织发现RBED1表达显著上调，其过表达促进癌细胞侵袭和EMT过程，机制可能与Wnt/β-catenin通路激活有关，提示RBED1作为潜在治疗靶点。
4. **文献名称**: "Optimization of RBED1 Recombinant Expression in E. coli for Functional Studies"
**作者**: Müller, S., et al.
**摘要**: 报道一种优化的大肠杆菌表达系统，通过密码子优化和融合标签策略提高RBED1可溶性表达，纯化蛋白适用于体外酶活性和抑制剂筛选实验。
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**注意**：以上文献为示例，实际研究中"RBED1"可能非标准命名，建议通过PubMed或UniProt核实基因/蛋白名称(如确认是否指“RBM家族”或类似RNA结合蛋白)。若需真实文献，请提供更准确的蛋白名称或基因符号。
**Background of Recombinant Human RBBP4 (RBED1) Protein**
RBBP4 (Retinoblastoma-Binding Protein 4), also known as RBED1 or NURF55. is a member of the WD40 repeat-containing protein family. It plays a critical role in chromatin remodeling and epigenetic regulation by interacting with histone deacetylases (HDACs) and polycomb group proteins. RBBP4 is a core component of multiple chromatin-modifying complexes, including the NuRD (Nucleosome Remodeling and Deacetylase) and PRC2 (Polycomb Repressive Complex 2), which are essential for histone modification, gene silencing, and cell cycle control.
Structurally, RBBP4 contains seven WD40 repeats that facilitate protein-protein interactions. Its ability to bind to histones, particularly unmodified H3 and H4. enables recruitment of chromatin regulators to specific genomic loci. Dysregulation of RBBP4 has been implicated in cancers, neurodevelopmental disorders, and aging-related diseases due to its role in maintaining chromatin stability and transcriptional fidelity.
Recombinant human RBBP4 is typically produced in *E. coli* or mammalian expression systems to ensure proper folding and post-translational modifications. It is widely used in studies exploring chromatin dynamics, gene regulation, and drug discovery targeting epigenetic pathways. Purification methods often involve affinity chromatography with tags (e.g., His-tag) followed by functional validation via binding assays. Research on RBBP4 continues to uncover its potential as a therapeutic target and biomarker in disease contexts.
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