| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBM12 |
| Uniprot No | Q9NTZ6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-932 aa |
| 活性数据 | MAVVIRLQGLPIVAGTMDIRHFFSGLTIPDGGVHIVGGELGEAFIVFATDEDARLGMMRTGGTIKGSKVTLLLSSKTEMQNMIELSRRRFETANLDIPPANASRSGPPPSSGMSSRVNLPTTVSNFNNPSPSVVTATTSVHESNKNIQTFSTASVGTAPPNMGASFGSPTFSSTVPSTASPMNTVPPPPIPPIPAMPSLPPMPSIPPIPVPPPVPTLPPVPPVPPIPPVPSVPPMTPLPPMSGMPPLNPPPVAPLPAGMNGSGAPMNLNNNLNPMFLGPLNPVNPIQMNSQSSVKPLPINPDDLYVSVHGMPFSAMENDVRDFFHGLRVDAVHLLKDHVGRNNGNGLVKFLSPQDTFEALKRNRMLMIQRYVEVSPATERQWVAAGGHITFKQNMGPSGQTHPPPQTLPRSKSPSGQKRSRSRSPHEAGFCVYLKGLPFEAENKHVIDFFKKLDIVEDSIYIAYGPNGKATGEGFVEFRNEADYKAALCRHKQYMGNRFIQVHPITKKGMLEKIDMIRKRLQNFSYDQREMILNPEGDVNSAKVCAHITNIPFSITKMDVLQFLEGIPVDENAVHVLVDNNGQGLGQALVQFKNEDDARKSERLHRKKLNGREAFVHVVTLEDMREIEKNPPAQGKKGLKMPVPGNPAVPGMPNAGLPGVGLPSAGLPGAGLPSTGLPGSAITSAGLPGAGMPSAGIPSAGGEEHAFLTVGSKEANNGPPFNFPGNFGGSNAFGPPIPPPGLGGGAFGDARPGMPSVGNSGLPGLGLDVPGFGGGPNNLSGPSGFGGGPQNFGNGPGSLGGPPGFGSGPPGLGSAPGHLGGPPAFGPGPGPGPGPGPIHIGGPPGFASSSGKPGPTVIKVQNMPFTVSIDEILDFFYGYQVIPGSVCLKYNEKGMPTGEAMVAFESRDEATAAVIDLNDRPIGSRKVKLVLG |
| 分子量 | 123.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人RBM12蛋白相关的参考文献摘要(因研究领域较细分，文献需通过数据库检索获取具体信息)：
1. **标题**：*RBM12 is a novel tumor suppressor in glioblastoma*
**作者**：Zhang Y, et al.
**摘要**：研究发现RBM12在胶质母细胞瘤中表达下调，体外实验表明重组RBM12通过调控RNA剪接抑制肿瘤细胞增殖和迁移，提示其作为抑癌基因的潜在功能。
2. **标题**：*Structural insights into the RNA-binding motif protein 12 (RBM12) in transcriptional regulation*
**作者**：Liu X, et al.
**摘要**：利用重组人RBM12蛋白进行冷冻电镜分析，揭示了其N端结构域与RNA/DNA结合的关键位点，并发现其可能通过结合特定RNA序列参与转录后调控。
3. **标题**：*RBM12 mutations alter RNA splicing in neurodevelopmental disorders*
**作者**：Johnson JL, et al.
**摘要**：全外显子测序发现RBM12基因突变与自闭症谱系障碍相关，体外表达重组突变体蛋白显示其RNA剪接功能异常，可能与神经发育缺陷相关。
注：以上文献标题和内容为虚拟概括，实际研究需通过PubMed/Google Scholar以"RBM12"、"RNA-binding protein"等关键词检索。建议补充具体研究需求(如疾病关联、分子机制)以进一步筛选文献。
RNA-binding motif protein 12 (RBM12) is a conserved eukaryotic protein belonging to the RNA-binding protein family, characterized by the presence of RNA recognition motifs (RRMs) and zinc finger domains. It plays a multifaceted role in RNA metabolism, including transcription, splicing, transport, and stability regulation. Studies suggest RBM12 interacts with spliceosome components and RNA polymerase II, influencing pre-mRNA processing and alternative splicing events that contribute to transcriptome diversity. Its involvement in the Wnt/β-catenin signaling pathway and cell cycle regulation highlights potential roles in development and cellular homeostasis.
RBM12 is widely expressed in human tissues, with elevated levels observed in certain cancers, linking it to tumor progression mechanisms. Notably, mutations in the RBM12 gene have been associated with neurodevelopmental disorders, implicating its critical function in neuronal development. Structural analyses reveal its ability to form liquid-liquid phase-separated condensates, a feature shared by many RNA-binding proteins involved in organizing cellular compartments for precise RNA processing.
Despite these insights, RBM12's precise molecular mechanisms and full range of biological functions remain under investigation. Its dual role in both normal physiology and disease contexts positions it as a compelling target for therapeutic exploration, particularly in oncology and neurodevelopmental research.
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