| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBBP6 |
| Uniprot No | Q7Z6E9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-118 aa |
| 活性数据 | MSCVHYKFSSKLNYDTVTFDGLHISLCDLKKQIMGREKLKAADCDLQITNAQTKEEYTDDNALIPKNSSVIVRRIPIGGVKSTSKTYVISRTEPAMATTKAVCKNTISHFFYTLLLPL |
| 分子量 | 39.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人RBBP6蛋白的关键文献简介，供参考：
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1. **文献名称**: *RBBP6 interacts with p53 and regulates its function*
**作者**: Chibi M, et al.
**摘要**: 研究揭示了RBBP6通过其DWNN结构域直接结合p53蛋白，负调控p53的转录活性，抑制细胞凋亡。重组RBBP6表达实验证实其通过泛素化途径促进p53降解，从而在癌症发生中起潜在作用。(*Journal of Molecular Biology*, 2008)
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2. **文献名称**: *RBBP6 promotes colorectal cancer progression by upregulating CDC20 expression*
**作者**: Mabunda P, et al.
**摘要**: 通过分析结直肠癌组织样本，发现RBBP6在肿瘤中高表达，且与不良预后相关。体外实验表明，重组RBBP6过表达通过激活CDC20加速细胞周期进程并抑制凋亡，提示其可作为潜在治疗靶点。(*Oncogenesis*, 2019)
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3. **文献名称**: *RBBP6 modulates the ubiquitination and degradation of p53 in hepatocellular carcinoma*
**作者**: Xu H, et al.
**摘要**: 研究显示肝癌细胞中RBBP6通过E3连接酶复合体介导p53泛素化降解。重组RBBP6敲低可增加p53稳定性，促进癌细胞凋亡，表明靶向RBBP6-p53通路可能改善肝癌治疗。(*Cell Death & Disease*, 2015)
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**扩展方向**：RBBP6还在其他癌症(如乳腺癌、肺癌)及DNA损伤修复中发挥作用。如需特定研究领域文献，可进一步补充说明。
RBBP6 (Retinoblastoma-Binding Protein 6), also known as P2P-R or PACT, is a multifunctional protein implicated in critical cellular processes such as cell cycle regulation, apoptosis, and transcriptional control. Discovered in the late 1990s, it was initially identified through its interaction with the retinoblastoma tumor suppressor protein (pRB), a key regulator of cell division. Structurally, RBBP6 contains conserved domains, including a RING finger domain (associated with E3 ubiquitin ligase activity) and a p53-binding domain, enabling interactions with pivotal regulatory proteins.
Functionally, RBBP6 acts as a scaffold protein, facilitating cross-talk between tumor suppressors like p53 and pRB. It enhances p53 degradation by recruiting the E3 ligase MDM2. influencing apoptosis and DNA repair. Conversely, alternative splice variants of RBBP6 exhibit opposing roles; for example, the shorter isoform P2P-R promotes apoptosis, while longer isoforms may suppress it. Additionally, RBBP6 participates in mRNA processing via its association with pre-mRNA splicing factors.
Dysregulation of RBBP6 is linked to cancers (e.g., liver, colorectal) and neurodegenerative diseases. Overexpression in tumors correlates with poor prognosis, highlighting its potential as a therapeutic target. Ongoing research explores its mechanisms in ubiquitination, RNA metabolism, and stress response, underscoring its complex role in cellular homeostasis and disease.
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