| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBBP5 |
| Uniprot No | Q15291 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-538 aa |
| 活性数据 | MNLELLESFG QNYPEEADGT LDCISMALTC TFNRWGTLLA VGCNDGRIVI WDFLTRGIAK IISAHIHPVC SLCWSRDGHK LVSASTDNIV SQWDVLSGDC DQRFRFPSPI LKVQYHPRDQ NKVLVCPMKS APVMLTLSDS KHVVLPVDDD SDLNVVASFD RRGEYIYTGN AKGKILVLKT DSQDLVASFR VTTGTSNTTA IKSIEFARKG SCFLINTADR IIRVYDGREI LTCGRDGEPE PMQKLQDLVN RTPWKKCCFS GDGEYIVAGS ARQHALYIWE KSIGNLVKIL HGTRGELLLD VAWHPVRPII ASISSGVVSI WAQNQVENWS AFAPDFKELD ENVEYEERES EFDIEDEDKS EPEQTGADAA EDEEVDVTSV DPIAAFCSSD EELEDSKALL YLPIAPEVED PEENPYGPPP DAVQTSLMDE GASSEKKRQS SADGSQPPKK KPKTTNIELQ GVPNDEVHPL LGVKGDGKSK KKQAGRPKGS KGKEKDSPFK PKLYKGDRGL PLEGSAKGKV QAELSQPLTA GGAISELL |
| 分子量 | 59.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RBBP5蛋白的3篇代表性文献示例(注：以下内容为虚构示例，实际文献需通过学术数据库核实)：
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1. **文献名称**：*Structural Insights into the Role of RBBP5 in MLL1 Methyltransferase Complex Assembly*
**作者**：Thiel, A.T., et al.
**摘要**：该研究解析了RBBP5与MLL1复合体的晶体结构，揭示了RBBP5通过N端结构域稳定复合体并增强组蛋白H3K4甲基转移酶活性的分子机制，为靶向表观遗传调控的疗法提供依据。
2. **文献名称**：*RBBP5 Is Essential for Hematopoietic Stem Cell Maintenance and Leukemogenesis*
**作者**：Zhang, P., et al.
**摘要**：通过基因敲除实验证明，RBBP5是维持MLL融合蛋白白血病细胞存活的关键因子，其缺失导致组蛋白甲基化异常和肿瘤生长抑制，提示其作为白血病治疗靶点的潜力。
3. **文献名称**：*Recombinant RBBP5 Expression and Functional Analysis in Epigenetic Regulation*
**作者**：Li, Y., et al.
**摘要**：报道了重组RBBP5蛋白在大肠杆菌中的高效表达与纯化方案，并验证其在体外促进组蛋白甲基转移酶复合体活性的功能，为相关药物筛选奠定技术基础。
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如需实际文献，建议在PubMed或Google Scholar检索关键词“RBBP5 structural biology”“RBBP5 cancer”“recombinant RBBP5 purification”等，或参考期刊如*Nature Structural & Molecular Biology*、*Cell*上的相关研究。
RBBP5 (Retinoblastoma-Binding Protein 5) is a conserved eukaryotic protein encoded by the RBBP5 gene in humans. As a member of the WD40-repeat protein family, it plays critical roles in epigenetic regulation, chromatin modification, and transcriptional activation. RBBP5 is best known as a core component of histone methyltransferase (HMT) complexes, particularly the MLL/SET1-family complexes (COMPASS-like), which catalyze histone H3 lysine 4 methylation (H3K4me) – a hallmark of active gene promoters. It functions as a scaffold protein, mediating interactions between catalytic subunits (e.g., MLL1) and other regulatory proteins like ASH2L and WDR5 to stabilize HMT complex assembly and enzymatic activity.
This protein also associates with chromatin-remodeling complexes like Polycomb Repressive Complex 2 (PRC2) and the NuRD complex, indicating its versatility in modulating gene expression. Structurally, RBBP5’s WD40 domains facilitate protein-protein interactions, enabling recruitment to specific genomic loci. Studies highlight its essential role in embryonic development, cell cycle regulation, and differentiation. Dysregulation of RBBP5 is linked to cancers, neurological disorders, and developmental anomalies due to its impact on oncogene silencing, DNA repair, and stem cell pluripotency. Therapeutic targeting of RBBP5-interacting pathways is an emerging area in precision oncology and epigenetic disease research.
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