| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RASSF2 |
| Uniprot No | P50749 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-326 aa |
| 活性数据 | MDYSHQTSLV PCGQDKYISK NELLLHLKTY NLYYEGQNLQ LRHREEEDEF IVEGLLNISW GLRRPIRLQM QDDNERIRPP PSSSSWHSGC NLGAQGTTLK PLTVPKVQIS EVDAPPEGDQ MPSSTDSRGL KPLQEDTPQL MRTRSDVGVR RRGNVRTPSD QRRIRRHRFS INGHFYNHKT SVFTPAYGSV TNVRINSTMT TPQVLKLLLN KFKIENSAEE FALYVVHTSG EKQKLKATDY PLIARILQGP CEQISKVFLM EKDQVEEVTY DVAQYIKFEM PVLKSFIQKL QEEEDREVKK LMRKYTVLRL MIRQRLEEIA ETPATI |
| 分子量 | 37.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人RASSF2蛋白相关的3篇参考文献的简要总结(基于公开领域知识模拟，非真实文献)：
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1. **文献名称**: "RASSF2 is a novel K-Ras-specific effector and a tumor suppressor"
**作者**: Vos, M.D., et al.
**摘要**: 该研究揭示了RASSF2作为Ras信号通路中的直接效应分子，能够与活化的K-Ras结合。通过重组RASSF2蛋白实验，发现其过表达诱导细胞周期阻滞和凋亡，提示其肿瘤抑制功能，并可能通过调控细胞增殖相关基因(如Cyclin D1)发挥作用。
2. **文献名称**: "Epigenetic inactivation of RASSF2 promotes cancer cell migration via dysregulation of the NF-κB pathway"
**作者**: Akhtar, S., et al.
**摘要**: 研究通过重组RASSF2蛋白的功能分析表明，其在多种癌症中因启动子甲基化而表达缺失。恢复RASSF2表达可抑制NF-κB信号通路的活性，降低肿瘤细胞侵袭能力，提示其在癌症转移中的调控作用。
3. **文献名称**: "Structural insights into RASSF2 interaction with microtubules and its role in apoptosis"
**作者**: Shivakumar, L., et al.
**摘要**: 该研究利用重组RASSF2蛋白进行结构解析，发现其通过C端结构域与微管蛋白结合，调控微管稳定性。实验表明RASSF2的缺失导致微管动力学异常，从而抑制凋亡信号传导，揭示了其维持基因组稳定性的分子机制。
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如需具体文献，建议通过PubMed或Google Scholar以“RASSF2 recombinant protein”、“RASSF2 function”等关键词检索近年来的高质量论文。
**Background of Recombinant Human RASSF2 Protein**
Recombinant human RASSF2 (Ras association domain family member 2) is a protein encoded by the *RASSF2* gene, a member of the RASSF tumor suppressor family. This family is characterized by Ras association (RA) domains and plays critical roles in regulating apoptosis, cell cycle arrest, and microtubule stability. RASSF2 contains a C-terminal SARAH (Sav/RASSF/Hpo) domain, enabling interaction with kinases like MST1/2 to modulate Hippo signaling, a pathway vital for organ size control and tumor suppression.
RASSF2 is frequently epigenetically silenced via promoter hypermethylation in cancers (e.g., colorectal, lung), linking its loss to tumor progression. As a scaffold protein, RASSF2 stabilizes microtubules, influences mitotic progression, and activates pro-apoptotic pathways by antagonizing oncogenic Ras signaling. Recombinant RASSF2. produced via expression systems like *E. coli* or mammalian cells, retains these functional properties, making it essential for *in vitro* studies to dissect its tumor-suppressive mechanisms. Research leverages recombinant RASSF2 to explore therapeutic strategies targeting epigenetic restoration or Hippo pathway activation in cancer, underscoring its potential in biomedical applications.
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