| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RASSF1 |
| Uniprot No | Q9NS23 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-344 aa |
| 活性数据 | SGEPELIEL RELAPAGRAG KGRTRLERAN ALRIARGTAC NPTRQLVPGR GHRFQPAGPA THTWCDLCGD FIWGVVRKGL QCARLSADCK FTCHYRCRAL VCLDCCGPRD LGWEPAVERD TNVDEPVEWE TPDLSQAEIE QKIKEYNAQI NSNLFMSLNK DGSYTGFIKV QLKLVRPVSV PSSKKPPSLQ DARRGPGRGT SVRRRTSFYL PKDAVKHLHV LSRTRAREVI EALLRKFLVV DDPRKFALFE RAERHGQVYL RKLLDDEQPL RLRLLAGPSD KALSFVLKEN DSGEVNWDAF SMPELHNFLR ILQREEEEHL RQILQKYSYC RQKIQEALHA CPLG |
| 分子量 | 39.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RASSF1蛋白的3篇代表性文献及其摘要内容：
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1. **文献名称**：*"RASSF1A suppresses cell proliferation and induces apoptosis through Hippo signaling pathway activation in lung cancer"*
**作者**：Zhou, X., et al.
**摘要**：研究通过重组表达RASSF1A蛋白，发现其能够激活Hippo信号通路，抑制肺癌细胞增殖并促进凋亡，揭示了RASSF1在肿瘤抑制中的分子机制。
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2. **文献名称**：*"Epigenetic inactivation of RASSF1A promotes bladder cancer progression by activating the mTOR pathway"*
**作者**：Wang, Y., et al.
**摘要**：文章利用重组RASSF1A蛋白进行功能恢复实验，证实RASSF1A基因的甲基化失活通过激活mTOR通路促进膀胱癌细胞迁移和侵袭，提出了靶向表观遗传修饰的治疗潜力。
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3. **文献名称**：*"Structural and functional characterization of the RASSF1 tumor suppressor protein"*
**作者**：Zhang, L., et al.
**摘要**：通过体外重组表达并纯化RASSF1蛋白，结合X射线晶体学分析其结构，揭示了其SARAH结构域在结合下游效应蛋白(如MST1)中的关键作用，为癌症治疗的靶点设计提供了依据。
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注：以上文献标题及内容为示例性质，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。建议结合关键词“recombinant RASSF1”或“RASSF1 expression”筛选近年研究。
The RASSF1 (Ras Association Domain Family Member 1) protein is a tumor suppressor encoded by the *RASSF1* gene, located on chromosome 3p21.3. a region frequently altered in cancers. It belongs to the RASSF family, which interacts with Ras GTPases to regulate cell cycle progression, apoptosis, and microtubule stability. RASSF1 exists as multiple splice variants, with RASSF1A and RASSF1C being the most studied. RASSF1A, the predominant isoform, is epigenetically silenced in many cancers through promoter hypermethylation, contributing to tumorigenesis by dysregulating pathways like Hippo, NF-κB, and apoptosis signaling. It stabilizes microtubules, facilitates mitotic arrest, and promotes pro-apoptotic signals through interactions with MST1/2 kinases. RASSF1C, less characterized, may have distinct roles, including modulating inflammatory responses.
Recombinant RASSF1 proteins are produced in *E. coli* or mammalian systems for functional studies, enabling exploration of their tumor-suppressive mechanisms, post-translational modifications, and interactions with oncogenic partners. Research on recombinant RASSF1 has highlighted its potential as a therapeutic target or biomarker for cancers, including lung, breast, and prostate malignancies. Its role in oxidative stress responses and genomic stability further underscores its relevance in cancer biology. Current studies focus on restoring RASSF1 expression or mimicking its function to counteract tumor growth and chemoresistance.
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