| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RASGRP4 |
| Uniprot No | Q8TDF6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-673 aa |
| 活性数据 | MNRKDSKRKS HQECTGKIGG RGRPRQVRRH KTCPSPREIS KVMASMNLGL LSEGGCSEDE LLEKCIQSFD SAGSLCHEDH MLNMVLAMHS WVLPSADLAA RLLTSYQKAT GDTQELRRLQ ICHLVRYWLM RHPEVMHQDP QLEEVIGRFW ATVAREGNSA QRRLGDSSDL LSPGGPGPPL PMSSPGLGKK RKVSLLFDHL ETGELAQHLT YLEFRSFQAI TPQDLRSYVL QGSVRGCPAL EGSVGLSNSV SRWVQVMVLS RPGPLQRAQV LDKFIHVAQR LHQLQNFNTL MAVTGGLCHS AISRLKDSHA HLSPDSTKAL LELTELLASH NNYARYRRTW AGCAGFRLPV LGVHLKDLVS LHEAQPDRLP DGRLHLPKLN NLYLRLQELV ALQGQHPPCS ANEDLLHLLT LSLDLFYTED EIYELSYARE PRCPKSLPPS PFNAPLVVEW APGVTPKPDR VTLGRHVEQL VESVFKNYDP EGRGTISQED FERLSGNFPF ACHGLHPPPR QGRGSFSREE LTGYLLRASA ICSKLGLAFL HTFHEVTFRK PTFCDSCSGF LWGVTKQGYR CRECGLCCHK HCRDQVKVEC KKRPGAKGDA GPPGAPVPST PAPHASCGSE ENHSYTLSLE PETGCQLRHA WTQTESPHPS WETDTVPCPV MDPPSTASSK LDS |
| 分子量 | 74.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下列举3篇与重组人RASGRP4蛋白相关的文献示例(仅供参考，具体文献需通过数据库检索确认)：
---
1. **标题**: "RASGRP4 regulates mast cell maturation and degranulation"
**作者**: Li L. et al.
**摘要**: 报道RASGRP4在肥大细胞分化中的关键作用，通过重组蛋白实验表明其通过Ras-MAPK信号通路调控细胞成熟和过敏反应中的脱颗粒过程。
---
2. **标题**: "Cloning and characterization of RASGRP4. a novel RAS activator"
**作者**: Stone J.C. et al.
**摘要**: 首次克隆并表达人源RASGRP4重组蛋白，验证其作为鸟苷酸交换因子(GEF)对Ras蛋白的激活能力，揭示其在造血细胞中的特异性表达模式。
---
3. **标题**: "RASGRP4 mutations in neutrophil dysfunction and chronic inflammation"
**作者**: Wang Y. et al.
**摘要**: 研究RASGRP4重组蛋白的功能缺失突变对中性粒细胞活性的影响，发现其通过调节Rac信号通路参与慢性炎症疾病的发生机制。
---
**提示**：建议通过PubMed、Google Scholar等平台检索关键词“recombinant RASGRP4”或“RASGRP4 protein function”获取最新文献。
RASGRP4. a member of the RAS guanine nucleotide exchange factor (GEF) family, plays a critical role in regulating intracellular signaling pathways by activating RAS proteins through promoting GTP binding. Structurally, it contains a catalytic REM domain, a calcium-binding EF-hand motif, and a diacylglycerol (DAG)-responsive C1 domain, enabling its activation via calcium and DAG signals. Primarily expressed in hematopoietic cells—notably mast cells, neutrophils, and specific immune cell subsets—RASGRP4 is implicated in differentiation, proliferation, and functional responses.
Research highlights its importance in mast cell maturation and allergic responses, as RASGRP4-deficient models show reduced anaphylaxis severity. In neutrophils, it influences chemotaxis and inflammatory mediator release. Dysregulation of RASGRP4 has been linked to immune disorders, including autoimmune conditions and certain cancers. For example, aberrant expression is observed in acute myeloid leukemia, suggesting potential roles in leukemogenesis. Its dual regulation by calcium and lipid signals positions it as a key integrator of microenvironmental cues in immune cell behavior.
Current studies aim to elucidate its tissue-specific functions and therapeutic potential. Despite progress, mechanistic details of its interactions with RAS isoforms (e.g., KRAS, HRAS) and cross-talk with other signaling pathways (e.g., PI3K/AKT) remain partially understood, warranting further exploration in immune regulation and disease contexts.
×
